Conlanging III: Words, Finally

Previously: Conlanging I: First Contact, Conlanging II: Contology, Hart's Nominex

Minor posts: Some affixes, mostly derivationalEvidentiality And Supposability

It's been a year since I've worked on my conlang for the Xenants and I think I'm ready to give them some words and bound morphemes. In the past, I worked on a lot on grammar and conceptual semantics, but now it's time to get this thing done. Lexicography. Lexiconning. Lexicoinage. Wordsmithing. Whatever.

:: Quick Review Of Language Features

: Speakers

The language doesn't have a name yet, but it's spoken by crystalline insectoid aliens called Xenants.

: Parts of Speech

The language of the Xenants has conjunctions, verbs, nouns, and lots of affixes (prefixes and suffixes). There are no stand-alone articles, adjectives, or adverbs.

: Phonology

Xenants bilaterally have two complex sound producing organs (we'll call them "mouths" here, but there's a different organ for ingestion that could also be called a mouth). They can produce sounds from mouths simultaneously, independently. Their sounds are highly percussive: Xenants don't have vowels or voicing, and they can't even whisper.

Xenants have the following sounds in their root vocabulary of affixes, nouns, verbs, and conjunctions:

O o (pi pu): the bilabial clicks ⟨ʘ⟩.
I i (ci cu): the dental clicks ⟨ǀ⟩.
T t (ti tu): the voiceless alveolar plosives ⟨t⟩.
Z z (zi zu): the voiceless alveolar affricates ⟨t͡s⟩.
X x (xi xu): the voiceless postalveolar affricates ⟨tʃ⟩.
K k (ki ku): the voiceless velar plosives ⟨k⟩.

All of those are distinguished into high and low forms (represented with capital case versus lowercase in the short 1-character transription, and represented by the vowel in the long romanization). Humans might be able to emulate the high and low forms by palatalization and rounding, respectively, and the vowel in the romanization help with that. The forms with the height specified are called consonants. If only the manner and place of articulation are specified, but not the height, then we're talking about an "articulant". A bilabial click for example, is an articulant to the Xenants, not a consonant.

The high and low forms are not related semantically, anymore than English "god" and "cot" are related semantically. Different sounds, different words, different meanings.

The names for the articulants (e.g. "the voiceless velar plosives") reference human anatomical features like teeth ("dental") and lips ("bilabial") that humans can use to approximate the Xenant sounds. There hasn't yet been any anatomical investigation of Xenant anatomy to figure out what organs are actually producing those sounds.

The Xenants also have two consonants used for saying numbers:
# . (ri ru): the aspirated voiceless alveolar taps ⟨ɾ̥ʰ⟩.

I haven't done anything with them yet, but I think the Xenants might also use these sounds non-linguistically. 

Sh : the voiceless palato-alveolar fricative ⟨ʃ⟩.
S : the voiceless alveolar sibilant ⟨s⟩.

A Xenant syllable consists of a consonant in one mouth, and silence or a consonant in the other mouth. Silence is transcribed with an underscore "_" in the short transcription and with an apostrophe "'" in the long romanization. Xenant syllables are called "half-syllables" if one mouth is silent, and "full-syllables" otherwise. Simultaneously produced syllables are written in sequence in both the short transcription scheme and the long romanization, so that "Xt" is a single syllable written short and "xitu" is the same syllable written long.

: Word Order

None. There is no required or suggested word order in the Xenant language. A given Xenants might have an artistic preference for a certain word order, perhaps introducing concepts in a given order for the sake of poetry or dramatic storytelling, but that's all. To be fair, there's a lot of synthetic morphologic structure within words, to the extent that it's more like Xenant words correspond to English phrases and the free Xenant word order is more like a free English phrasal order, but that's not unusual for natural synthetic languages either.

: Phonotactics

For the most part, the Xenant language is designed so that Xenants don't have to speak the same articulant in one mouth on two successive syllables ("stuttering"). This held true throughout the entire language until recently when I decided on words for phrasal conjunctions.

Let's have an example: we've already seen the syllable "xitu". If a Xenant were presented with this syllable twice in a row, it might say the first xi in its left mouth and the second xi in its right mouth, and likewise the consonant "tu" would alternate mouths. Almost every word and morphological construction pattern in the language is designed around giving Xenants options like that to swap sounds left and right to avoid stuttering. And maybe I should fix the conjunctions to avoid stuttering also. We'll see.

That's it for review. Now for some new words!

:: Phrasal Conjunctions

Conjunctions are the roots of Xenant discourse. They link together all the other simple sentences. They will be single syllable words, and the syllables will all have doubled consonants (i.e. shared articulants and shared height). There are twelve such syllables and here they are with their twelve corresponding conjunctions:

First, logical and causal conjunctions:

"OO": AND
"oo": BUT
"II": AND-OR
"ii": EITHER-OR
"TT": IF-THEN
"tt": AS-SO

. Next, conjunctions of relative temporal reference (tensive and aspectual conjunctions):

"ZZ": BEFORE
"zz": AFTER
"XX": SINCE
"xx": UNTIL
"KK": WHILE
"kk": WHEN

.

By themselves, there's no phonotactic problem with these words as shown above, but conjunctions don't occur bare: they incorporate morphemes ("indexical proforms") that are used to reference the simple sentences over which the conjunctions operate. An indexical pro-form incorporated into a conjunction can point to a verb at the root of a simple sentence or to a conjunction with its own arguments. The sequence of syllables after this incorporation can violate the phonotactic constraint against stuttering. For example, if the incorporated morpheme has a Z, then you can't stick on the conjunction BEFORE ("ZZ") without stuttering. Not ideal.

I'm not super happy with that violation, but I wanted to do something important with the doubled consonant syllables and using them as function-words like conjunctions feels good. Also, I'm really happy with the set of 12 conjunctions here as a complete set that fits in the fixed space of 12 doubled syllables, so for now I'll shrug off the phonotactic violation and content myself with the knowledge that it doesn't happen more than once per word? Or I could add a new sound to the language just for conjunctions, maybe, to give an bilateral alternation option. Or .... I'll figure something out.

First thought: conjunctions are two syllables long now, and the second syllable has (a high alveolar tap and silence). I haven't used that syllables anywhere else in the language, and we'll see later together that it's a pretty logical way of announcing that indexical proforms are coming next in the word. So now the conjunction AND would look like "OO#_". Honestly, this only feels slightly better than violating the stutter rule. I'll keep thinking.

! How about a hyphen pronounced as a silent beat? Silence denotes word separation, except after conjunctions, where it denotes a hyphen. Yeah. I can deal with that. Problem solved.

:: Verbs

The Xenant language only has two root verbs. They're both half-silent syllables:

"O_": EXIST
"I_": CAUSE

. I might get rid of CAUSE at some point, but it's here for now. And that's okay. It's good, even, maybe. Existence, the universe, is a great directed acyclic graph of causal interactions; the two verbs are both very ontologically fundamental. I don't feel bad about having both, even if the language might be more impressive with just one or the other. It wouldn't be better, I don't think, it would just be more constrained.

Root verbs get lots of affixes to become complex words that basically correspond to simple sentences in English, so you'll often see me calling affixed verbs "phrases" or "simple sentences", but such a sentence is really just one word for the Xenants. Also, if you read my previous posts, you'll see me called affixed nouns "verb phrases", because they're like English phrases and they're verbs to the Xenants, but they're not verb phrases in the sense that they don't have a subject. Oops. Misleading. I might go back and edit that out.

:: Numbers and indexical proforms

Xenant numbers were described and spelled in "Conlanging I", but let's review them here. Xenants use base 12. The integers 0 through 11 are: "O#", "I#", "T#", "Z#", "X#", "K#", "o#", "i#", "t#", "z#", "x#", "k#", where the sharp sign represents a high aspirated voiceless alveolar tap.

The duodecimals 0/12 through 11/12 are: "O.", "I.", "T.", "Z.", "X.", "K.", "o.", "i.", "t.", "z.", "x.", "k.", where the period represents a low aspirated voiceless alveolar tap.

The Xenants have a positional number system just like ours, but they use #-numerals ahead of the duodecimal point and .-numerals after the duodecimal point. Also, they don't have a duodecimal point. So, e.g., "O#I#T.Z." looks like 

(1) (2) (3/12) (4/12) 

in sequence, but positionally it means

1*(12^1) + 2*(12^0) + 3*(12^-1) + 4*(12^-2)

, better known as 

12 + 2 + 3/12 + 4/144 = 14 and 40/144ths

. I haven't figured out how to represent negative numbers yet or anything more exotic, but we'll get there in time.

Positive integers are really important in the Xenant language, which has a completely free word order. Integers are used pronomially (referring to nouns) and pro-verbally (referring to simple sentences that are semantically-headed by verbs) and pro-conjunctively (referring to conjunctions) and these integers are incorporated into verbs and conjunctions, and that's how the Xenants show relationships between words, instead of using sequential syntax. These integer proforms are also indexical: a number 1 incorporated into a verb references the 1st noun in the sentence. A number 3 incorporated into a conjunction references the 3rd verb or conjunction mentioned in the sentence. In total, conjunctions reference verbs and other conjunctions, and verbs reference nouns.

Incorporation is just concatenation, if that sounded complicated. A conjunction IF-THEN that references verb 1 and verb 2 might just look like: IF-1-THEN-2. Easy peasy. Except they would write it short as "I#-TT-T#", maybe, I'm still not sure about the morpheme order, and also there wouldn't be dashes, I just thought you might like some help with parsing.

If you're having trouble pronouncing that, try the romanization: ciri-titi-tiri. If possible, try splitting it up as a Xenant would do; say ci-ti-ti in your dominant mouth and ri-ti-ri in your non-dominant mouth. I believe in you.

:: TAME Verbal affixes (Tense, Aspect, Mood, Evidentiality)

I haven't said much about how verbal affixes are spelled. In previous posts, I decided that nominal affixes would be two syllables long, and in particular, nominal prefixes would be a half syllable followed by a full syllable (-hf), and nominal suffixes would be a full syllable followed by a half syllable (-fh). Furthermore, those full syllables should have distinct articulants, i.e. they should come from the set ["OI", "OT", "OZ", "OX", "OK", "Oi", "Ot", "Oz", "Ox", "Ok", "IT", "IZ", "IX", "IK", "Io", "It", "Iz", "Ix", "Ik", "TZ", "TX", "TK", "To", "Ti", "Tz", "Tx", "Tk", "ZX", "ZK", "Zo", "Zi", "Zt", "Zx", "Zk", "XK", "Xo", "Xi", "Xt", "Xz", "Xk", "Ko", "Ki", "Kt", "Kz", "Kx", "oi", "ot", "oz", "ox", "ok", "it", "iz", "ix", "ik", "tz", "tx", "tk", "zx", "zk", "xk"]. I'll often refer to these as "boundary syllables". This is a huge space of affix morphemes and there's definitely room to fit all the verbal and nominal affixes I could want in it. But also, as I'm imagining what Xenant sentences will look like, I'm seeing that the verbs, when fully affixed and incorporated, are really really long in comparison to the nouns and conjunctions, and I'd like to try shortening them a little, if possible. So maybe I'll make the TAME verbal affixes be half syllables, and all other verbal affixes (i.e., adverbial and adpositional affixes) will look like the two-syllable nominal affixes.

Picking half-syllables for the TAME affixes would be a lot easier if I felt confident in which ones I wanted, and easier still if I were sure about how many (half-syllable) verbs the language should have. Here's my first stab at it:

: Tense suffixes for verbs:

(past): "k_"
(present): "x_" (default, usually unmarked)
(future): "z_"

: Aspect suffixes for verbs:

(perfective): "t_" (default, usually unmarked)
(continuous): "i_
(inceptive): o_
(terminative): K_

: Mood suffixes for verbs:

(realis): "X_" (default, usually unmarked)
(counterfactual): "Z_"
(generic/gnomic): "T_"

.

And I guess I'll skip the evidential affixes for lack of space, even though my evidential affix system was so fucking good:

Perceptive: noticeably
Weak perceptive: seemingly
Ductive (Deductive/Inductive): logically/reasonably
Weak ductive: theoretically, speculatively, hypothetically
Quotative: reportedly
Weak quotative: allegedly, anecdotally

. Maybe those will be two-syllable affixes. Yeah. Maybe. ... Nah, I'll put it in another language.

Let's try an example!

Let's say that some object (indexed with the number 1) really existed in the past, and we want to talk about it's existence at the time as an ongoing state or process, not an event. All together that's [exist] [#1] [tense=past] [aspect=continuous] [mood=realis]. We have words and/or morphemes for all of those parts! In particular, they correspond to [O_] [I#] [k_] [i_] [∅]. So "it was existing'" is rendered as "O_I#k_i_". I'm not sure about the morpheme order, and I'm especially unsure where the indexical pronoun numbers will incorporate, but still, we have the parts! Yay!

Here's the same word spelled long: pi'-ciri-ku'-cu'.

:: Nouns

In the Xenant language, root nouns with no affixes will start and end with boundary syllables, i.e. full syllables that don't have the same articulant. If I use just one boundary syllable for very general nouns, then it's still starting and ending with a boundary syllable, so that's how I'll start. Here are some general nouns paired up completely randomly with different boundary syllables. 

Entity: ot
Material: Zx
Element: zk
Chemical: Tk
Silicate mineral: Kz
Non-silicate mineral: OX
Material form: Xi
Inanimate natural object: OI
Rock: tk
Artefact: it
Organism: IZ
Organism part: tz
Agent: Ix
Social group: TX
Composition: IX
Massed Cognitive Endurant: Ot
Counted Cognitive Endurant: Ik
Directed part: Tx
Shape: Ox
Place: xk
Time period: ik
Polar Perdurant: ox
Massed Manner Perdurant: XK
Counted Manner Perdurant: Xo
Absolute spatial stative dimension: ix
Relative spatial stative dimension: Zk
Absolute abstract stative dimension: iz
Relative abstract stative dimension: To
Absolute spatial dynamic dimension: Kt
Relative spatial dynamic dimension: oz
Absolute abstract dynamic dimension: ZX
Relative abstract dynamic dimension: IT
Formal unit: OT
Informal unit (collection): Oz

And just for my own use, here are the boundary syllables that I haven't used as noun categories / ontological phonosthemes: ["OZ", "OK", "Oi", "Ok", "Io", "It", "Iz", "TZ", "TK", "Ti", "Tz", "Zo", "Zi", "Zt", "Zk", "Xt", "Xz", "Xk", "Ko", "Ki", "Kx", "oi", "ok", "tx", "zx", "xk"].

There's still room for many more general nouns like that, if I want to expand things, and I do. But for now, we can speciate those general nouns into specific nouns. For example, organism, "IZ", could speciate into organism roles such as familial relations, e.g. (parent child ancestor descendant mate sibling) or into organism types like (bacterium plant animal). I'd like to start with the familial relations. How about a half syllable "x_" in the middle to say that we're speciating into familial organism roles and then a random boundary syllable at the end will give us a big space for all the relations to have their own words.

Parent : IZx_Kx
Child : IZx_IZ
Ancestor : IZx_Xz
Descendant : IZx_ix
Grandparent : IZx_Xk
Grandchild : IZx_ot
Mate : IZx_ZX
Sibling : IZx_ok
Cousin : IZx_Tz
Nibling : IZx_To
.

Now let's speciate organism parts! I'll speciate separately for different biological kingdoms. The Xenant planet has intelligent large motile things which we could call animals, perhaps. Animal body parts will get "O_" as a middle half syllable.
  

The general noun "organism part" was "tz". Let's now introduce: 

Brain : tzO_ik
Leg : tzO_Zk
Thorax : tzO_Zi
Abdomen : tzO_zx
Anus : tzO_ZX
Foot : tzO_OX
Throat : tzO_Ki
Tooth : tzO_Ik
Stomach : tzO_TX
Head : tzO_OI
Mouth : tzO_Tx
Maw : tzO_Ot

Xenant mouths are for talking and Xenant maws are for ingesting.

The general noun "composition" was "IX". Again let's stick a second boundary syllable on there directly in order to speciate:
Word : IXZX
Name : IXXo
Sentence : IXXt
Message : IXKi
Record : IXZk
Aphorism : IXoi
Rule : IXXK
Contract : IXOz
Design : IXIx
Language : IXOT
Algorithm : IXIT
Program : IXtx
Game : IXZt
Explanation : IXot
Prediction : IXIk
.

I had such a hard time coming up with words in the past and now they're all coming out at once. It feels good, man. I think the Xenants use the same word as a program, IXtx, to mean a model, as in "we have a model of the problem". And they use the same word for an algorithm, IXIT, to mean a method/strategy/technique, as in "this my method of chopping root vegetables". I was tempted to say that the word for an explanation or the word for a prediction should also be used for a program execution or a simulation, but maybe that's a type error - the latter two are perdurants (at least, if not also endurants by polysemy), and we'll treat them separately later. However, the word for an explanation, IXot, is the same for hypotheses and theories. Also, the word for prediction, IXIk, isn't limited to a future context: it's also used for estimation in the present and post-diction.

I tried coming up with some English common-names for plants of the Xenant homeworld. I think their words probably won't be noun+noun and adjective+noun combinations like these, but I couldn't just say "there are 100-ish Xenant plants and they all have short gibberish names". They might have plants like ...
acid root
ant bane
ant-bird berry
antlion bite
antlion eggs
antlion grass
aphid bush
aphid-bird berry
bird grass
bitter suckle
blow berry
box leaf
braid wood
broad leaf
brush wood
chick foot
chick grass
cold moss
collar stem
cone flower
dead vine
dimpled stem
doodlebug wood
dwarf bud
fast vine
feather flower
fiber wood
fin leaf
fine seed
flat vine
flea seed
float berry
float seed
foot leaf
genital weed
gold root
grass flower
ground berry
hair stem
hard bean
heart flower
heat bush
hemolymph root
iron root
itch weed
larva bane
larva nettle
lion-bird berry
long bloom
lower-mantle cone flower
luck bush
magma grain
magma tree
mound cover
mound weed
needle flower
needle fruit
nickel wood
oil bean
oil tree
pheromone fruit
pin leaf
poison berry
poison tuber
poison wood
potassium berry
pungent root
rock flower
round leaf
sand fruit
sap flower
sap stalk
screw bean
sessile seed
sharp weed
silica wood
silver wood
sinking spinel
small flower
soft bean
soft leaf
soft weed
spice berry
spiky worm seed
stimulant plant
sting suckle
sweet tuber
sweet wood
sword leaf
tall stem
thorium berry
thorium nectar
three ants
throat vine
tooth flower
tuber wood
uranium berry
valley flower
wandering poison
weeping flower
wild root
worm wood
.
They'll all get Xenant words eventually. Ooh, maybe I can make short gibberish plant names for use in conversation and then long names like those above for use in poetry.

I mentioned that the Xenants are crystalline. That's not fully true. The hard parts of their bodies are mostly crystals, but they've got semi-liquid silicones and silanes as a medium for most of their biochemistry. Still, they have lots of words for minerals. Many more words than I can fit into two full syllables, or even a full syllable, a half syllable, and another full syllable. I could make all minerals three syllable words, but not all minerals are of equal importance to the Xenants, and things of common importance should be assigned shorter words. So I think I'm stuck with a situation where minerals XY and XYZ won't necessarily be related, except that they're both Xs. I'm not going to bore you with their words for minerals, but I think that XY versus XYZ thing I just said is an important fact about the language, and I wanted to document why I'm making that choice. It's because there are a ton of minerals. If you *do* want to read all about minerals, I have a semi-categorized list of ~194 minerals that are important here on earth, and the Xenants probably have words for similar things to those, although skip the ones with carbon, and maybe the hydrated minerals would be replaced with anhydrous versions, and the Xenants would also have many more minerals that are rich iron, magnesium, and zinc. and also some more minerals rich in chromium, vanadium, manganese, cobalt, and copper.

No, I'm better than that. Here are some silicate minerals that Xenants have words for (which are also likely to occur in the upper or lower mantle of the earth):

Tectosilicates:
KzTx: stishovite (tetragonal SiO2)
Kziz: coesite (monoclinic SiO2)
Garnets:
KzOZ: almandine (cubic Fe3Al2(SiO4)3)
KzIX: pyrope (cubic Mg3Al2(SiO4)3)
KzTz: spessartine (cubic Mn3Al2(SiO4)3)
KzIo: andradite (cubic Ca3Fe2(SiO4)3)
KzTK: grossular (cubic Ca3Al2(SiO4)3)
Kzix: uvarovite (cubic Ca3Cr2(SiO4)3)
KzXt: majorite (cubic Mg3(MgSi)(SiO4)3)
Pyroxenes:
Kzzk: aegirine (monoclinic NaFeSi2O6)
KzTo: jadeite (monoclinic NaAlSi2O6)
KzTk: hedenbergite (monoclinic CaFeSi2O6)
Kzzx: diopside (monoclinic MgCaSi2O6)
KzOi: clinoenstatite (monoclinic MgSiO3)
KzTZ: clinoferrrosilite (monoclinic FeSiO3)
Olivines:
KzIK: forsterite (orthorhombic Mg2SiO4)
KzIx: fayalite (orthorhombic Fe2SiO4)
Spinel-group silicate minerals:
KzOt: ringwoodite (cubic Mg2SiO4)
Kzoi: ferroringwoodite (cubic Fe2SiO4)
Ilmenites:
KzZK: ferro-Akimotoite (trigonal FeSiO3)
Kzot: magnesio-Akimotoite (trigonal MgSiO3)
Silicate perovskites:
KzXk: davemaoite (cubic CaSiO3)
Kzxk: bridgmanite (orthorhombic MgSiO3)
KzTX: ferro-bridgmanite (orthorhombic FeSiO3)
Miscellaneous neosilicates:
KzOT: zircon (tetragonal ZrSiO4)
.
.

The Xenants also have words for non-silicate minerals, including:  
Oxides:
periclase: cubic MgO
wüstite: cubic FeO
magnetite: cubic Fe3O4
hematite: trigonal Fe2O3
maghemite: cubic Fe2O3
pyrite-FeO2: cubic FeO2
bunsenite: cubic NiO
xenantite: orthorhombic Ni2O3
corundum: trigonal Al2O3
cassiterite: tetragonal SnO2
cuprite: cubic Cu2O
uraninite: cubic UO2
rutile: tetragonal TiO2
Non-silicate spinel-group minerals:
spinel: cubic MgAl2O4
hercynite: cubic FeAl2O4
magnesio-ferrite: cubic MgFe2O4
trevorite: cubic NiFe2O4
chromite: cubic FeCr2O4
magnesio-chromite: cubic MgCr2O4
Non-silicate perovskites:
FeAlO3-bridgmanite: orthorhombic FeAlO3
AlAlO3-bridgmanite: orthorhombic AlAlO3
.
.
"No diamond (cubic C), James?", you ask me. I'm not sure. Not yet. It's fun to think of a world without carbon.

I'd really like to include some more nickel minerals. These are all rare on the surface of the earth and I'm not sure about their existence/abundance at greater depths:

nickeline: hexagonal NiAs
gersdorffite: cubic NiAsS
pecoraite: monoclinic Ni3(Si2O5)(OH)4
népouite: orthorhombic Ni3(Si2O5)(OH)4

And these are also rarely found on the surface, but they have possible substitutions in their formulas, whereas I've only been giving names to end-members of solution series, so they'll probably all get separated into a pure nickel mineral and a pure non-nickel mineral:

nickeliferous goethite: orthorhombic (Fe,Ni)O(OH)
nickeliferous akaganeite: monoclinic (Fe,Ni)O(OH)
falcondoite: orthorhombic (Ni,Mg)4Si6O15(OH)2·6H2O
pentlandite: cubic (Fe,Ni)9S8
.

Finally there are some iron-nickel alloys commonly found in chondrite meteorites with definite crystal structure but indefinite or non-integral stoichiometry. I'm going to pretend that the stoichiometry is one-to-one and call these ones minerals also:

taenite/kamacite: cubic FeNi
tetrataenite: tetragonal FeNi
.
Xenants have words for those too. Cool.

There are lots of other minerals that are familiar to me from the surface of earth, but I don't know if they're likely to be prevalent within a rocky planet, so by default I'll make them 3 syllables long. Honestly, the very first one on this list, acanthite, is a very good theoretical candidate for something the Xenants might know; it contains silver, which is a dense metal, and sulfur, which must be present in some significant quantity in the mantle or it wouldn't be so strongly associated with volcanism. But I've never read about acanthite in a source on mantle geochemistry, so for now, it gets three syllables.   

acanthite: monoclinic Ag2S
albite: triclinic NaAlSi3O8
alum-K: cubic KAl(SO4)2.12H2O
alum-Na: cubic NaAl(SO4)2.12H2O
alunite: trigonal KAl3(SO4)2(OH)6
analcime: cubic NaAlSi2O6.H2O
anatase: tetragonal TiO2
andalusite: orthorhombic Al2SiO5
anglesite: orthorhombic PbSO4
anhydrite: orthorhombic CaSO4
anorthite: triclinic CaAl2Si2O8
antarcticite: trigonal CaCl2.6H2O
anthophyllite: orthorhombic Mg7Si8O22(OH)2
aragonite: orthorhombic CaCO3
arcanite: orthorhombic K2SO4
arsenopyrite: monoclinic FeAsS
atacamite: orthorhombic Cu2Cl(OH)3
augelite: monoclinic Al2(PO4)(OH)3
autunite: orthorhombic Ca(UO2)2(PO4)2.10-12H2O
azurite: monoclinic Cu3(OH)2(CO3)2
baryte: orthorhombic BaSO4
beryl: hexagonal Be3Al2(SiO3)6
bischofite: monoclinic MgCl2.6H2O
bobierrite: monoclinic Mg3(PO4)2.8H2O
boehmite: orthorhombic AlO(OH)
borax: Na2B4O5(OH)4.8H2O monoclinic
bornite: orthorhombic Cu5FeS4
brochantite: monoclinic Cu4(OH)6SO4
brookite: orthorhombic TiO2
brucite: trigonal Mg(OH)2
brushite: monoclinic CaHPO4.2H2O
calcite: trigonal CaCO3
celestine: orthorhombic SrSO4
cerussite: orthorhombic PbCO3
chalcanthite: triclinic CuSO4.5H2O
chalcocite: monoclinic Cu2S
chalcopyrite: cubic CuFeS2
chloroapatite: hexagonal Ca5(PO4)3Cl
chrysoberyl: orthorhombic BeAl2O4
cinnabar: trigonal HgS
clinochrysotile: monoclinic Mg3Si2O5(OH)4
clinozoisite: monoclinic Ca2Al3(Si2O7)(SiO4)O(OH)
coffinite: tetragonal USiO4
colemanite: monoclinic CaB3O4(OH)3.H2O
covellite: hexagonal CuS
cristobalite: tetragonal SiO2
diaspore: orthorhombic AlO(OH)
dioptase: trigonal CuSiO2(OH)2
dolomite: trigonal CaMg(CO3)2
enstatite: orthorhombic MgSiO3
epidote: monoclinic Ca2Al2Fe(SiO4)(Si2O7)O(OH)
epsomite: orthorhombic MgSO4.7H2O
esseneite: monoclinic CaFeAlSiO6
ettringite: trigonal Ca6Al2(SO4)3(OH)12.26H2O
euclase: monoclinic BeAlSiO4(OH)
fluorite: cubic CaF2
fluoroapatite: hexagonal Ca5(PO4)3(OH)
galena: cubic PbS
ghiaraite: triclinic CaCl2.4H2O
gibbsite: monoclinic Al(OH)3
glauberite: monoclinic Na2Ca(SO4)2
glaucophane: monoclinic ()Na2(Mg3Al2)Si8O22(OH)2
goethite: orthorhombic FeO(OH)
graphite: hexagonal C
greenockite: hexagonal CdS
grunerite: monoclinic Fe7Si8O22(OH)2
gypsum: monoclinic CaSO4.2H2O
halite: cubic NaCl
halloysite: monoclinic Al2Si2O5(OH)4
hanksite: hexagonal Na22K(SO4)9(CO3)2Cl
hausmannite: tetragonal Mn3O4
hemimorphite: orthorhombic Zn4Si2O7(OH)2.H2O
hoelite: monoclinic C14H8O2
howlite: monoclinic Ca2B5SiO9(OH)5
hydroxyapatite: hexagonal Ca5(PO4)3F
hypercinnabar: hexagonal HgS
ilmenite: trigonal FeTiO3
jarosite: trigonal KFe3(SO4)2(OH)6
kaolinite: triclinic Al2Si2O5(OH)4
kyanite: triclinic Al2SiO5
langite: monoclinic Cu4(OH)6SO4.H2O
lawsonite: orthorhombic CaAl2Si2O7(OH)2.H2O
leucite: tetragonal KAlSi2O6
litharge: tetragonal PbO
lizardite: trigonal Mg3(Si2O5)(OH)
magnesite: trigonal MgCO3
malachite: monoclinic Cu2(OH)2CO3
manganite: monoclinic MnOOH
massicot: orthorhombic PbO
mesolite: orthorhombic Na2Ca2(Al2Si3O10)3.8H2O
metacinnabar: cubic HgS
microcline: triclinic KAlSi3O8
millerite: trigonal NiS
mirabilite: monoclinic Na2SO4.10H2O
molybdenite: hexagonal MoS2
monetite: triclinic CaHPO4
nacrite: monoclinic Al2Si2O5(OH)4
nahcolite: monoclinic NaHCO3
natrolite: orthorhombic Na2Al2Si3O10.2H2O
natron: monoclinic Na2CO3.10H2O
newberyite: orthorhombic MgHPO4.3H2O
niter: orthorhombic KNO3
nitrocalcite: monoclinic Ca(NO3)2.4H2O
orpiment: monoclinic As2S3
orthoclase: monoclinic KAlSi3O8
otavite: trigonal CdCO3
pennantite: triclinic Mn5Al(AlSi3O10)(OH)
petalite: monoclinic LiAlSi4O10
portlandite: hexagonal Ca(OH)2
prehnite: orthorhombic Ca2Al(AlSi3O10)(OH)2
pyrite: cubic FeS2
pyrochroite: trigonal Mn(OH)2
pyrolusite: tetragonal MnO2
pyromorphite: hexagonal Pb5(PO4)3Cl
pyrophyllite: monoclinic Al2Si4O10(OH)2
quartz : trigonal SiO2
realgar: monoclinic As4S4
rhodochrosite: trigonal MnCO3
riebeckite: monoclinic Na2Fe5Si8O22(OH)2
rutherfordine: orthorhombic UO2(CO3)
sanidine: monoclinic KAlSi3O8
scheelite: tetragonal CaWO4
scorodite: orthorhombic FeAsO4.2H2O
sepiolite: orthorhombic Mg4Si6O15(OH)2.6H2O
siderite: trigonal FeCO3
sillimanite: orthorhombic Al2SiO5
sinjarite: tetragonal CaCl2.2H2O
smithsonite: trigonal ZnCO3
soda niter: trigonal NaNO3 
sodalite: cubic Na8(Al6Si6O24)Cl2
spodumene: monoclinic LiAl(SiO3)2
staurolite: monoclinic Fe2Al9O6(SiO4)4(OH)2
stibnite: orthorhombic Sb2S3
stilbite-Ca: monoclinic NaCa4(Si27Al9)O72·28(H2O)
stilbite-Na: monoclinic Na9(Si27Al9)O72·28(H2O)
strengite: orthorhombic FePO4.2H2O
strontianite: orthorhombic SrCO3
struvite: orthorhombic (NH4)MgPO4.6H2O
sylvite: cubic KCl
syngenite: monoclinic K2Ca(SO4)2.H2O
talc: monoclinic Mg3Si4O10(OH)2
tenorite: monoclinic CuO
tephroite: orthorhombic Mn2SiO4
thenardite: orthorhombic Na2SO4
titanite: monoclinic CaTiSiO5
tremolite: monoclinic Ca2Mg5Si8O22(OH)2
troilite: hexagonal FeS
trona: monoclinic Na3(CO3)(HCO3).2H2O
turquoise: triclinic CuAl6(PO4)4(OH)8.4H2O
ulexite: triclinic NaCaB5O6(OH)6.5H2O
uranophane: monoclinic Ca(UO2)2(SiO3OH)2.5H2O
vivianite: monoclinic Fe3(PO4)2.8H2O
willemite: trigonal Zn2SiO4
witherite: orthorhombic BaCO3
wollastonite: triclinic CaSiO3
wulfenite: tetragonal PbMoO4
xenotime: tetragonal YPO4
zoisite: orthorhombic Ca2Al3(SiO4)(Si2O7)O(OH)
.
A dozen of those have carbon. Oops. 

I've mentioned that I prefer to use end-member minerals in preference to solution-series, but here are are some famous minerals with substitutions that the Xenants might also have names for:

actinolite: monoclinic Ca2(Mg, Fe)5Si8O22(OH)2
andesine: triclinic (Ca, Na)(Al, Si)4O8
anorthoclase: triclinic (Na, K)AlSi3O8
augite: monoclinic (Ca, Na)(Mg, Fe, Al, Ti)(Si, Al)2O6
biotite: monoclinic K(Mg, Fe)3(AlSi3O10)(F, OH)2
chabazite: triclinic (Ca, K2, Na2)2[Al2Si4O12]2.12H2O
chamosite: monoclinic (Mg, Fe)5Al(Si3Al)O10(OH)8
chondrodite: monoclinic (Mg, Fe)5(SiO4)2(F, OH)2
clinochlore: monoclinic (Mg, Fe)5Al(Si3Al)O10(OH)8
clinoptilolite: monoclinic (Na, K, Ca)2-3Al3(Al, Si)2Si13O36.12H2O
cordierite: orthorhombic (Mg, Fe)2Al4Si5O18
cummingtonite: monoclinic (Mg, Fe)7Si8O22(OH)2
ferrosilite: orthorhombic (Mg, Fe)2Si2O6
hornblende: monoclinic Ca2(Mg, Fe, Al)5(Al, Si)8O22(OH)2
hypersthene: orthorhombic (Mg, Fe)SiO3
illite: monoclinic (K, H3O)(Al, Mg, Fe)2(Si, Al)4O10[(OH)2, (H2O)]
labradorite: triclinic (Ca, Na)(Al, Si)4O8
lazulite: monoclinic (Mg, Fe)Al2(PO4)2(OH)2
lazurite: cubic (Na, Ca)8(S, Cl, SO4, OH)2(Al6Si6O24)
lepidolite: monoclinic K(Li, Al)3(Al, Si, Rb)4O10(F, OH)2
monazite: monoclinic (Ce, La)PO4
muscovite: monoclinic KAl2(AlSi3O10)(F, OH)2
palygorskite: monoclinic (Mg, Al)2Si4O10(OH).4H2O
phlogopite: monoclinic KMg3(AlSi3O10)(F, OH)2
pigeonite: monoclinic (Ca, Mg, Fe)(Mg, Fe)Si2O6
rhodonite: triclinic (Mn, Fe, Mg, Ca)SiO3
scorzalite: monoclinic (Fe, Mg)Al2(OH, PO4)2
sphalerite: cubic (Zn, Fe)S
thorite: tetragonal (Th, U)SiO4
topaz: orthorhombic Al2SiO4(F, OH)2
vesuvianite: tetragonal Ca10(Mg, Fe)2Al4(SiO4)5(Si2O7)2(OH, F)4
wavellite: orthorhombic Al3(PO4)2(OH, F)3.5H2O
whitlockite: trigonal Ca9(Mg, Fe)(PO4)6PO3OH
wolframite: monoclinic (Fe, Mn)WO4
wurtzite: hexagonal (Zn, Fe)S
.
And now you know 250 of my favorite minerals. I sure hope they in the minerrological community come up with better names for pyrite-FeO2 and the end-members of the bridgmanite series soon.

Let's speciate material forms next. Xenants have 18 short words for them.

XiOt: gas in solid solution (interstitial hydride)
XiIT: gas in solid suspension (sponge)
XiTx: liquid in solid solution (amalgam)
Xitz: liquid in solid suspension (gel)
XiXt: solid in solid solution (solid)
XiKz: solid in solid suspension (aggregate)
Xiix: gas in liquid solution (seltzer)
XiZo: gas in liquid suspension (foam)
XiOx: liquid in liquid solution (liquid)
Xiit: liquid in liquid suspension (emulsion)
XiOZ: solid in liquid solution (syrup)
XiZk: solid in liquid suspension (sol)
Xiox: gas in gas solution (gas)
XiOT: gas in gas suspension ()
Xiot: liquid in gas solution ()
XiIz: liquid in gas suspension (mist)
XiIt: solid in gas solution ()
XiOI: solid in gas suspension (smoke)
.
The entries with empty parentheses don't have short English names, probably because they don't exist. I think the word for a solid in solid suspension, XiKz, "aggregate", probably deserves further speciation, but I'm not sure whether the descendant words will have two or three syllables, and if three syllable, then whether they will start with XiKz. Words like sand and powder and ...

:: Sentences and conversations:

Here's a cute idea. I've said that Xenants use lexical indices as an infinite family of incorporated proforms, and that the indices count from the start of a sentence, but I've never defined where a sentence ends or how the Xenants mark it. Maybe the indices should instead be specific to the conversation, so that a second Xenant can use the same proforms as the first one to refer to the same concepts. And then if you want to start over counting, you say something like "Zero!" and the conversation starts fresh. Even more Xenantishly, they could say, "Every word exists as zero suddenly.".

:: Adverbial, adpositional, and derivational, verbal affixes

Affixes are prefixes and suffixes and infixes. I think all my verb affixes are going to be suffixes, but we'll take them one at a time.

: Adverbial suffixes

I've cleaned up my set adverbial suffixes from Conlanging I. They are now:

* Progress and speed: (increasingly | decreasingly), (suddenly | gradually)
* Frequency: (never | constantly) (once | repeatedly) (rarely | frequently)
* Telicity: (intentionally | unwillingly | stupidly | unintentionally)

The affix with the English gloss "repeatedly" that contrasts with "once" can be used for a single repetition or many, so another possible gloss for the affix would be "again". 

The Xenants think of the adverbial suffixes of telicity as lying in a two dimensional space:

* Forethought (+), Choice (+): Intentionally
* Forethought (+), Choice (-): Unwillingly
* Forethought (-), Choice (+): Stupidly
* Forethought (-), Choice (-): Unintentionally

. They don't have a word "accidentally" that can be used just as well for things you did stupidly as for things that you did unintentionally. "Stupidly" might sound a little jarring to you; it certainly does to me. You might wonder if instead, maybe we could give the suffix a polite English gloss like "imprudently"? But no. The Xenants are jarring. They call each other stupid and they mean it.

I guess I need to make morphemes for these suffixes now! Suffixes in the language usually have the two-syllable form (-fh), where "f" is a full syllable with sounds in both mouths, followed by "h", a half syllable for which one mouth is silent. The full syllable is also a "mixed" or "boundary" syllable, i.e. the two mouths aren't saying the same consonant, since those syllables with shared consonants are only used for conjunctions. This lets the Xenants avoid stuttering.

The suffixes of Progress and Speed will all have OZ for their full syllables:

OZO_: Increasingly
OZK_: Decreasingly
OZz_: Suddenly
OZT_: Gradually

Frequency suffixes will have "IZ" for the full syllable:

IZT_: Never
IZX_: Constantly
IZi_: Once
IZx_: Repeatedly
IZZ_: Rarely
IZo_: Frequently

. And the telic adverbial suffixes will start with "TZ'": 

TZX_: Intentionally
TZi_: Unwillingly
TZz_: Stupidly
TZK_: Unintentionally

. Cool. None of these suffixes are required to be present and none of them take arguments. You just slap them on to the end of verbs, as many as you want, in whatever order you want, though the order of application might change the meaning, especially when composed with other suffixes. Semantically, they modify everything that's come before them. So in comparing, "exist-suddenly-as-a-child" and "exist-as-a-child-suddenly", the first one might mean to come into existence knock-kneed and upright but immature, while the second one might mean to become a child after having previously been an adult. Those are both weird impossible situations, but sometimes Xenants need to write about weird impossible situations, like in literature.

Fair warning: if you add on antonymic suffixes in a contradictory way, like to say that something happens at once both gradually and suddenly, a Xenant listener might attack you, unless you're very young and very close kin.

: Adpositional verbal affixes

Xenants have two kinds of adpositional verbal suffixes: spatial and thematic. And the spatial affixes also come in two types: locative or directive. And I'm not sure about any of them.

- Thematic Adpositions

Let's start with the thematic adpositions. When I say "thematic" I'm gesturing at thematic roles, like "agent", "patient", "instrument", "recipient", which live in the realm of semantics, and they're closely related to syntactic nominal cases, like the "nominative" and the "ergative". In practice, I don't know much about thematic roles or syntactic nominal cases, but it seems to me that they're often introduced with prepositions in languages that don't mark nouns for case, and that's basically how Xenants introduce them also. The difference is that the prepositions are suffixes on verbs that take arguments, and the arguments are numerical references to nouns, counted from their position in the sentence. My thematic affixes are suffixes (postpositional rather than prepositional), but I don't yet feel confident in what they will be. In decreasing order of usefulness, I've mainly considered:

* Similative : "as"
* Possessive: "with"
* Pertinitive: "about"
* Instrumental: "by (means of)"
* Benefactive: "for"
.

To say that a given book is old, a Xenant would say "the-book an-old-thing exists-1-as-2", or any rearrangement of those three words, with the numbers changing to match, e.g. "exists-2-as-1 an-old-thing the-book".

In practice, I think the first three thematic affixes ("as", "with", "about") will usually go on the EXIST verb and the other two ("by", "for") will mostly go on the CAUSE verb, but doing otherwise wouldn't necessarily be ungrammatical.

I've given the pertinitive suffix a one word English gloss of "about". Originally I used it in situations where an English speaker would say "pertaining to", and "about" is pretty close to that and shorter. I also find myself using it in cases where an English speaker would say "w.r.t." / "with respect to" or "in the context of". I'm not super positive that all four of those quoted glosses are close synonyms. Maybe one of the meanings should be factored out for semantic regularity.

I'd considered some other (pro | anti)-benefactive thematic suffixes, with glosses like "in spite of", "so that", "in order to", "lest", but I'm leaning against including them now. They don't seem all that useful, and when I want to express things like that, I'd rather do it with multiple verbs linked by conjunctions.

I'd really like to use "that" as a thematic affix, in sentences like "he existed with a belief that (x)" or "the article existed with a claim that (x) " or "I exited with a desire that (x)". But its not clear to me that "that" is being used as a preposition there. Linguistics call it it a "complementizer" for introducing a sentential complement, but that doesn't necessarily mean it's not a preposition. I think it probably isn't a preposition, but the fact that linguistics have another name for it doesn't contribute to that. The best alternative I'd come up with to using "that" as a thematic affix was using uncommon nouns like "positive information" and "positive desire". "He existed with positive information about (x) (being the case)" or "He existed with positive desire about (x) (being the case)". Not great, right?'

Another problem with using "positive X" constructions that I've just noticed, besides that I'm resorting to using new weird nouns to say normal things, is that the sequential application of affixes doesn't correspond to sequential modification of meaning. In "he existed with positive desire about x", we're reading the "about x" like it's is modifying "positive desire", because that makes sense, but if affixes modify everything that came before them, then it should be modifying "he existed with positive desire". His positive desire wasn't about x, but rather his existence with positive desire was about (x). Does that ....change anything? I'm so tired. Maybe expressing this should be done using two existence simple sentence, like "A positive desire existed about X (being the case)" and "he existed with 1".

This is super important. The ability to make sentential complements is basically equivalent to the ability to quote language within language. And quotation, the ability to talk about language, is almost as basic as the existence of language. I need this.

Okay, new idea, which might actually be an old idea from Conlanging I, as I can't remember everything I've written. I've talked in other posts about derivational affixes that can turn sentences into perdurants, like "the dog exists with a tail" becomes "the state of the dog existing with a tail". With this derivational affix, we could start with the English sentence "He believed that the dog had a tail." and transform it to something more Xenantish like "He existed with a belief and the belief existed as (the state of the dog existing with a tail)." But we can do better! Let's have a derivational affix that turns sentences of the language into quoted sentences. Then we can say "He existed with a belief and the belief existed as (the dog exist with a tail)-sentence". I like that way more. Beliefs exist as sentences. I stand by that. And desires, on the other hand, they exist "for" perdurants, benefactively. "He desired to be free." becomes "He existed with a desire" for the first part and "the desired existed for (he would-exist-as a-free-thing)-state". This is good. I feel like I've brought out semantic features that would have been hidden if I'd just used "that" willy-nilly.

So let's create some morphemes for the for thematic/adpositional suffixes. They seem pretty adequate now that I've got an idea of how to combine them with derivational affixes to compose sentences where English would use sentential complements. I'm still not super confident that I'll stick with the exact five suffixes that I mentioned above, but I doubt the final set will have more than six members.

Let's use "iz" for the full syllable. Also, the gloss "by" for the Instrumental thematic suffix always sounds wrong without tacking on the parenthetical, in oder to give us "by (means of)", so let's use a different gloss. "Through" sounds okay. "He caused the storm to exist through machinery." Stuff like that.

izZ_: Similative suffix, "as"
izt_: Possessive suffix, "with"
izx_: Pertinitive suffix, "about"
izi_: Instrumental suffix, "through"
izO_: Benefactive suffix, "for"

The Xenants really like organizing things into opposites and these aren't organized like that, which is sad, but maybe I'll have an insight down the line that will let me redo this Xenantishly. In the mean time, they're too useful not to use.

- Spatial Adpositions

We've had thematic adpositinal verbal suffixes, which look like prepositions. There are lots of other prepositions, and a lot of them look like the conjunctions we've already covered, the logical, the causal, and the temporal. The remainder of the prepositions are pretty much spatial (orientating and/or directive). Also the word "of" is a big important remaining preposition. We'll get to "of" later. There will be a whole section on genitive nominal relations.

There's a commonly drawn distinction in linguistics wherein languages are said to have verb-framing versus satellite-framing based on whether the location/path of a motion verb is directly encoded in the verb or whether it get stuck into a separate particle, like a preposition. Germanic languages generally have satellite framing using prepositions (go in, go out). Romance languages generally have verb-framing, with different verbs for different motions and no preposition needed (enter, exit). English has a mixture of both due borrowings of Norman French into Old English. Romance languages have a habit of using a second gerund of a verb in an adverbial manner after a directive verb to specify a manner ("he exited running").

The Xenant language, of course, encodes both the path and the manner separately from the root verb (CAUSE or EXIST), when it encodes them at all. Slobin calls languages like this "equipollently-framed", which is a delicious word and almost as much fun to say as "Slobin". What affixes do the Xenants use for location and direction?

Xenant spatial suffixes come in two sets of six suffixes. All of them take an argument, which we'll call a reference object. Members from the first set are used when we're not considering any boundary of the reference object,

(near-to | far-from)
(parallel-to | perpendicular-to)
(with | opposite)
.

The second set of six suffixes basically share their semantics with the fist set element-wise, except that they're used when the reference object is a boundary or has a boundary, and also they suggest but don't require contact with the boundary.

(within | outside-of)
(around/along | through/across)
(astride | against)

. In the second pair of antonyms, "(along | across)" are my English glosses for the suffixes when the reference boundary is long/linear and "(around | through)" are glosses for the suffixes when the reference boundary is not, but the Xenants use the same suffix in either case.

Those two sets are used by Xenants to talk about fixed position and orientation. To talk about motion, Xenants usually just tack on the verbal modifiers "(increasingly | decreasingly)", so that e.g. motion (toward | away from) a reference object is expressed as being (near to | far from) it -increasingly. Likewise entrance and exit can be expressed as being (within | outside of) something -increasingly.

Time to invent some great new morphemes! It's mighty morpheme time.

tkK_: near-to
tki_: far-from
tkt_: parallel-to
tkz_: perpendicular-to
tkZ_: with
tkk_: opposite

IoK_: within
Ioi_: outside-of
Iot_: around/along
Ioz_: through/across
IoZ_: astride
Iok_: against

The half syllables are the same element-wise between the unbounded frame and the boundary-contact frame. Also, the unbounded frames have the same full syllable (tk) and the bounded frames have the same full syllable (Io). Other than that, there's no structure, i.e. (with | opposite) are antonyms but their half syllables were chosen semi-independently / randomly without replacement.

I could have given all 12 spatial affixes the same first syllable, since there are 12 half syllables available to distinguish them, but I like this better. It shows more structure and it gives me space in case I want to add more spatial affixes.

: Derivational verbal affixes

Derivation changes a word in a deeper way that inflection, often by changing the word's part of speech. I've already talked about two (verbal to nominals) derivational affixes in this post: one that makes affixed verbs / simple sentences into perdurants (i.e. nouns X about which we can say "it happened during X) and one that quotes an affixed verb / simple sentence so that language can reference composed language, e.g. when talking about what someone said. Those two are all I care to make at the moment. Here they are in the Xenant language:

zxk_: (makes a perdurant from an affixed verb)
zxO_: (quotes an affixed verb)

The Xenant language has a huge inventory of nouns, so there aren't a ton of situations where you need to make up nouns from other parts of speech, but these two are good examples.

I think that's it for verb affixes! They were all suffixes! Surprise! What should we do next? Speciate more verbs? Nah, not just yet. Let's dive into nominal affixes! I hardly touched on that in previous posts. This will be new ground.

I'd like to pause for a second and say that it was just within this post that I solved conjunctions, adverbial affixes, and spatial adpositions, and I did an amazing job. They are really Xenanty now. It's entirely possible that "Xenanty" will enter the English language just because I did such a good job on these, and I am to be commended.

:: Nominal affixes

: Determiners

Determiners will be nominal prefixes. We haven't done any prefixes yet, so the choice of sounds is completely free. They'll have the "hf-" syllable pattern, with a half syllable followed by a full syllable.

- Articles, Quantifiers, Distributive Determiners

Xenants don't have possessive determiners for now. Maybe they don't have a concept of possession. Someone is in control of things at a moment and that's as close to ownership as they get. They do have articles and quantifiers, which I'm presenting at once because I've never really understood the difference. The same articles and quantifiers are used on mass nouns, singular count nouns, and plural nouns. I've gotten a few so far, grouped them into pairs, and given them functional titles as best as I can: 

Small (non-existential) definite quantifier: No/None-Of
Large (universally-existential) definite quantifier: All/All-of-the

Small existential indefinite quantifier: A/An/Some/Some-of/At-least-one
Large existential indefinite quantifier: Most/Most-of/All-but-some

Small median indefinite quantifier: Little/Few/Little-of
Large median indefinite quantifier: Much/Many/Much-of
.

I think "little/few" allows for the possibility of referencing zero things, but "a/some" doesn't. "Some" means at least one. The (few | many) pair is more confusing to me than than the other quantifiers. I don't know that it guarantees anything definite about the number or proportion of referenced items. I think they're relative to an expected/typical or perhaps adequate quantity, so I've called them "median" quantifiers.

Let's make up some morphemes. Let's stick all of these on the same full syllable "Oi".

t_Oi: No
k_Oi: All
X_Oi: Some
o_Oi: Most
z_Oi: Few/Little
Z_Oi: Many/Much

The Xenants don't have a word for "the". They also don't have dedicated prefixes that correspond to the English distributive determiners "Any" or "Each/Every". To say "I don't like any of these dogs", you just say "I like none of these dogs.". To say "I want to hug each of these dogs.", you just say "I want to hug all of these dogs.". Speaking of "these" dogs, ...

- Demonstrative Determiners

Xenant demonstrative determiners come in (proximal, medial, distal varieties) and also (singular, plural) varieties. The full-syllable for them is "Ik".

t_Ik: proximal singular (this) X_Ik: proximal plural (these) k_Ik: medial singular (that) x_Ik: medial plural (those) Z_Ik: distal singular (yon thing) o_Ik: distal plural (yon things)

. I think Xenants use singular and plural for mass nouns like substances when they're distinguishable. If some of your gold is slightly more dense, Xenants will say "these golds" rather than "this gold".

I think the Xenants might also use the proximal / medial / distal distinction idiomatically to refer to 1st, 2nd, and 3rd persons. The sentence "We speakers are talking to you listeners about their crimes." will be rendered by a Xenant more like "These speakers are talking to those listeners about the crimes of yon people.".

The closest thing Xenants have to a first person singular pronoun is the three syllable word t_IkIx, which means "this agent", although that word also has non-idiomatic usage for referencing a proximal agent. Like you can talk about "this dog" without calling yourself a dog, you see.

...

I don't like that. I want something else. Let's see what we can brainstorm. How about a distinction between reflexive, proximal, and medial?

t_Ik: reflexive singular (itself)
X_Ik: reflexive plural (themselves) k_Ik: proximal singular (this) x_Ik: proximal plural (these) Z_Ik: medial singular (that) o_Ik: medial plural (those)
.

No, that's not good either. To say "the man killed himself", Xenants just reference the man twice, they don't mention a man and then mention him reflexively. So ... So screw it. Let's remove number information from the demonstrative determiners, add in three possessive determiners and then have number be expressed separately.

t_Ik: proximal (this/these)
k_Ik: medial (that/those)
Z_Ik: distal (yon thing(s))
X_Ik: proximal (my / our)
x_Ik: medial (your)
o_Ik: distal (their)

.

Actually, this still isn't better. To talk about yourself, "this agent" still sounds better than "my agent", so to talk about other people, it seems regular to keep saying "that agent" instead of "your agent" to talk about the listener in a conversation.

...

Oh, hey, whoa, Xenants wouldn't mark nouns for grammatical number (e.g. using affixes), because changes of grammatical number will change a noun's ontological type, and Xenantish nouns tell you their ontological type with the first prefix of the root. "One dog" is an organism, "two dogs" is a set of organisms. I need an ontological type for collectives, and then a genitive construction to say "collective the members of which are type dog". That sounds a little unwieldy, but it's what the Xenants would do. There isn't any point having ontological phonesthemics if we're not going to respect it. 

Unless if when they say something that sounds to us like "one dog" they mean "a set containing one dog"?

...

New idea. All Xenant nouns are uncountable mass nouns, like furniture and luggage and fauna. When they write something we translate as "one animal" it's semantically more like "one piece of fauna". Um..... To sick to think things.

- Interrogative Determiners

Xenants don't have interrogative determiners like "what", "which", "whose". They also don't ask questions. They're good at making threats though. That's one way they get information out of each other. 

- Cardinal Numerals

If I'm putting quantifiers like ("no", "all", "some") on nouns as affixes, it makes sense to also put on numbers like (1, 12, 144). The Xenant's way to say "144 dogs" is "144-dogs".

What if you want to talk about multiple dogs, without specifying the exact number or their (proximal, media, distal) relation to you. How do Xenants do that? I don't think they do. If you want to talk about dogs generically, like "Dogs are loyal.", that's what the generic/gnomic mood in the TAME verbal suffixes is for; "Dog(s) exist-generically as a loyal thing". Ah, but what if you want to say "half of the dogs" and not "half of the dog"? Then you have to specify how close it is to you. Sorry, bod. I know it's not very English, but this language isn't English.

: Genitive Suffixes

The word "of" in English has lots of functions. Even when we just talk about it linking nouns, it does so in many different ways. "Book of gold" is made of gold, "Book of Mormon" is written by Mormon, "book of the month", et. cetera. I'll call them all genitives. When other people are talking, I usually think of ownership, composition, and parthood as the prototypical genitive relations, but there are tons of noun-to-noun relationships, and I'll call them all genitive here

Here are some noun-to-noun relationships of common importance that I'd like the Xenants to be able to express.

Origin, Family, and Composition:

* X that is from Y.
* X that was made by or by means of Y.
* X that is a part or member of Y
* X that is made of Y.

Function and Purpose:

* X that is used on Y
* X that makes Y.
* X that destroys Y.
* X that seeks or collects Y.

Situational association:

* X that is used by Y.
* X that belongs at Y.
* X that affects Y.
* X that contains or is laden with Y.

I keep on going back and fourth as to whether it's okay to have two variables X and Y like above. The other option is to just have X be "thing" and Y is the only variable. A little more concretely, I'm waffling between genitive affixes of the form "X that has Y" versus "Thing that has Y". Even more concretely, I'm wondering whether I want affixed nouns to look more like "Box that has holes" versus "Thing that has holes".

Let me explain a little bit. If the genitive suffixes have one variable, Y, then I can turn "gold" into "a thing made of gold" and express "gold book" with a sentence like "a-book exists-as a-thing-made-of-gold". If the genitive modifies a base noun X and incorporates a reference to a noun Y, then I can say "some-gold a-book-made-of-1", which is like a compound noun, sort of.

With two variables, it feels like I'm sneaking in verbs, like "affects" and "seeks" and "makes", and I don't want nouns to relate to each other through those. Nouns relate to nouns through verbs, and the language's verbs are "exist" and "cause".

On the other hand, indexical numerals already incorporate into conjunctions and verbs. It's symmetrical/regular for them to also incorporate into nouns. Also also, I wanted the base noun's first syllable to tell you the word's ontological category, like how the Xenant words for "parent", IZx_Kx, and "grandchild", IZx_ot, both start out with the organism phonostheme, IZ. If I use the one variable version of the genitives, then gold, a substance beings "thing made of gold", an artefact, which breaks the ontological phonosthemics. So at the moment, I'm leaning toward two variable genitives, even though they feel a little impure.

I'm going to give each of the three sets of genitive affixes their own full consonant, but I've rearranged them like twelve times and nothing every feels very systematic, so the current groups probably aren't right and will hopefully change in the future when I figure out a better organization scheme.

There's no reason not to use the same boundary syllables in an affix that we've used in root nouns, and with these ones, I'm going to be a little cute. I'm going to use "xk" to start morphemes for the members the Origin/Family/Composition set, which is also the Xenant word for "place". I'll use "it" to start the morphemes from the Function/Purpose set, and "it" is also the Xenant word for "artefact". I'll use "ox" as the full syllable for the genitives of situational association, and "ox" is also the Xenant word for "event". I feel like I might be inviting people/Xenants to make nominal interpretations of the other affixes by doing this, which is bad, and also it's it of character for the language since it's cute and Xenants aren't cute .... buuuuut I'm still doing it.

And here we are: 12 genitive affixes for making compound nouns:

xkz_: that is from
xkO_: that was made by or by means of
xkk_: that is a part or member of
xko_: that is made of

itI_: that is used on
itO_: that makes
itK_: that destroys
itk_: that seeks or collects

oxI_: that is used by
oxZ_: that is found at or belongs at
oxK_: that affects
oxt_: that is laden with

. Cool. 

I'm having a little trouble remembering which full syllables I've used for suffixes and prefixes. Let's take stock:

Adverbial suffixes: (OZ, IZ, TZ)
Thematic adpositional suffixes: (iz)
Spatial adpositional suffixes: (tk, Io)
Verbal to nominal derivational suffixes: (zk)
Quantifying prefixes: (Oi)
Demonstrative prefixes: (Ik)
Genitive suffixes: (xk, it, ox)
.

It's totally fine for a suffix and a prefix to have the same full syllable, it just hasn't happened yet. The morphemes "hf-" and "-fh" simply don't have the same meaning, for any values of "f" and "h".

:: Adjectival nouns

The language of the Xenants doesn't have adjectives, but it has lots of nouns for binary/antonymic dimensions. I think the binary dimensions come in eight families. I'm not sure if they have multiple words for the dimension, coming both from the high end of the scale and the low end of the scale, e.g. whether they have words for both "emptiness" and "fullness" or just one of the two. Here are the eight families with some common/important members listed, but not their antonyms.

* Spatial stative, absolute: unity openness emptiness size length width height straightness sharpness smoothness
* Spatial stative, relative: presence coverage distance inversion neatness alignment

* Abstract stative, absolute: complexity rarity normality uniformity newness
* Abstract stative, relative: relevance dependence sufficiency necessity value equivalence compliance attractiveness completeness

* Spatial dynamic, absolute: activity radioactivity hotness intensity mobility speed tension tightness heaviness actinopacity viscosity noisiness turbulence
* Spatial Dynamic, relative: powerfulness stability fragility hardness rigidity

* Abstract dynamic, absolute: autonomy intelligence consciousness healthiness liveliness maturity
* Abstract dynamic, relative: functionality sensitivity preparedness harmfulness knowledgeability confidence attentiveness productivity efficiency

"Actinopacity" means "particle radiation stopping power". The Xenants are nuclear radiotrophs and they think that concept should be described in one word, not four.

Yeah, let's make dimension-words for both ends of the spectrum. That's Xenantish for two reasons: 1) Xenants like opposites and symmetries and regularity, and 2) they rely on a large inventory of nouns to express themselves given their small closed classes of conjunctions, verbs, and maybe affixes, so more nouns are generally welcome. Also, writing down both antonyms might occasionally make it clearer to you, my dear reader, which dimension I'm trying to point at. Also also, it takes a lot of thought and motivational opportunity for me to make progress on language design features like "dimensions should come in these eight clusters", and once I've done that design work, I'm going to get out as many words out of the design constraints as I can.

* Absolute spatial stative dimension: ix
ixik: unity
ixOX: division / separation
ixix: openness
ixtx: closedness
ixTK: largeness
ixZi: smallness
ixOK: length
ixXo: shortness
ixoz: width
ixXi: narrowness
ixOT: height/depth
ixZk: lowness/shallowness
ixIZ: straightness
ixTi: deviation/deflection
ixtz: sharpness
ixTX: dullness
ixZt: roughness
ixZX: smoothness

* Relative spatial stative dimension: Zk
ZkOX: presence
ZkTi: absence
Zkox: coverage
ZkIk: bareness
ZkTo: emptiness
ZkIx: fullness
ZkOt: distance
ZkOT: nearness
ZkOz: inversion
ZkTZ: uprightness
ZkZt: neatness
ZkOZ: disorganization
Zktz: alignment
Zkik: misalignment

* Absolute abstract stative dimension: iz
izZt: complexity
izTi: simplicity
izIZ: rarity
izTK: commonness
izox: normality
izxk: unusualness
izXz: uniformity
iziz: diversity
izKi: newness
izZx: oldness

* Relative abstract stative dimension: To

ToIt: relevance
ToIo: irrelevance
ToKz: dependence
ToIK: independence
ToZK: sufficiency
Toiz: insufficiency
ToIZ: necessity
Toit: inessentiality
ToXo: value
ToXt: worthlessness
ToOz: equivalence
ToTZ: inequivalence
ToZi: compliance
ToTi: non-compliance
ToIk: attractiveness
ToOX: unattractiveness
ToOZ: completeness
ToXi: incompleteness

* Absolute spatial dynamic dimension: Kt
KtOk: activity
KtTo: inactivity
Kttz: radio-inactivity
KtKx: hotness
KtZK: coldness
Ktox: intensity
KtXt: mildness
KtIX: mobility
Ktix: immobility
KtXo: speed
Ktzx: slowness
KtZo: tension
KtIZ: looseness
Ktoi: compression
KtIK: expansion/noncompressedness
Kttk: heaviness
KtTi: lightness
KtIo: actinopacity
Ktot: actinoclarity
KtKo: viscosity
KtKz: fluidity
KtOt: noisiness
KtXz: quietness
KtIk: turbulence
Kttx: fluid-lamination

* Relative spatial dynamic dimension: oz
ozXK: powerfulness
ozIo: feebleness
oziz: stability
oztz: instability
ozOZ: toughness
ozTk: fragility
ozKi: rigidity
ozit: flexibility
ozZX: hardness
ozIz: softness

* Absolute abstract dynamic dimension: ZX
ZXIk: autonomy
ZXIZ: heteronomy
ZXIK: intelligence
ZXOt: stupidity
ZXXo: consciousness
ZXZi: unconsciousness
ZXzk: health
ZXKt: sickness
ZXZo: liveliness
ZXiz: death
ZXtk: maturity
ZXIX: neoteny

* Relative abstract dynamic dimension: IT
ITOK: sensitivity
ITOi: insensitivity
ITzx: knowledgeability
ITTz: ignorance
ITOt: confidence
ITOz: uncertainty
ITTx: attentiveness
ITZx: inattentiveness
ITOX: functionality/capability
ITOT: non-functionality/incapability
ITTZ: efficiency
ITKo: inefficiency
ITot: harmfulness/destructiveness
ITKt: safety
ITIx: productivity
IToz: non-productivity
.

There is no nominal genitive affix for linking regular nouns with the properties/dimensions they exemplify. If you want to say that a person produces a lot of art, you say "person productivity art 1-exists-with-2-pertaining-to-3". For the absolute adjective classes, you can just say things like "1-exists-with-2". For the relative adjective classes, Xenants expect another verbal argument, like "-pertaining-to-3"

...

:: Perdurants

Holy crow, this language is so close to done, and it keeps mostly getting better and not worse, which is not like most things I do. Super happy. I just need to talk about perdurants, and then roles, and then flesh out/speciate the remaining phonosthemes a little, and then a section on Xenant writing systems, and then a section on their aphorisms, and then their creation myth, and then rewriting all of this so it looks more like a professional reference grammar, and then posting it literally everywhere online, including your mom's DMs.
 
If you can talk about things happening during a noun, that noun is a perdurant (in contrast to an endurant that fully exists at a given time). Things like weddings, famines, spaceship launches, and sneezes. More generally, states, events, processes, and activities.

Lots of perdurants are polar: they come in opposite pairs. Things can happen during someone's absence or their presence. Things can happen during an opening or a closure. Things can happen during a degradation or an improvement. I came up with a tidy list of important polar perdurants in Conlanging II, and we'll look at the evolution of that shortly.

Lots of other perdurants don't come in pairs. For example, noise and silence might be antonymic perdurants, but specific bodily sounds like (burp, cough, hiss, hum, laugh, shout, sigh, slurp, snarl, snore, snort, yelp) generally don't have opposites. I'd like to call these "manners" (of sound production) or manner perdurants. We'll treat them differently in the language that the polar perdurants. 
There are other manners, like manners of contact and manners of motion. Indeed, sounds are produced by motion and contact, so manners of sound production are kind of a special case of those. Social perdurants and cognitive perdurants also often don't have opposites, We'll talk more about all of them shortly.

Back to polar perdurants. About 85% of the polar perdurants I want to talk about can be easily expressed using just the two verbs, the adverbial affixes, and the polar dimensions that we just defined in the last section. The Xenants could still have separate short words for the perdurants, but I really want to see if I can be a little more clever and express all 100% of the polar perdurants by combining other language features. I think that will help me to make the other features a little more complete. And then if I still want separate words for the polar perdurants, I'll be able to define them all in the language, like a sample from a Xenant dictionary.

Mmm, not much progress with the last 15%. Let's just try expressing the rest in a dictionary-like way.

You can talk about things happening during activity and during inactivity, so those are perdurants. They were also polar dimensions, so that's easy:

activity-(perdurant) := some-entity activity-(dimension) exist -continuously 1 -with 2

And here it is spelled out:

activity-(perdurant) := X_Oiot KtOk O_i_I#izt_T#zxk_

 . I get a little sad when see the language spelled out with the short transcription. It just looks like line noise. The long romanization is much better:

activity-(perdurant) := xi'picuputu kitupiku pi'cu'ciricuzutu'tiri

It looks, you know, vaguely Austronesian or something.

...

I don't want to write them all out. Expansion/growth means existing increasingly with size. Taking means causing something to start not existing with a processed object. (Burying | excavation) mean causing something to start existing (within | outside of) the ground. Xenants can talk about restraining versus unbinding in terms of causing something to exist with (mobility | immobility), and they can talk abut (capture | release) in terms of causing something to exist with (autonomy | heteronomy). The perdurants of (introduction | phaseout) might just be expressed as (cause to exist -inceptive-aspect | cause to exist -terminative-aspect). There are just tons and tons of perdurants that can be expressed with the verbal affixes and the polar dimensions - so many that it might be more interesting to talk about the ones that can't.

The pairs of (hostility | hospitality/pleasantry), (assistance | attack), (cooperation | competition) are all quite social and I think the first two pairs might be worth combining completely, but I don't know how to express them. We have "with | against" among the verbal affixes, but those were spatial/directive frames and using them here would be metaphorical, which isn't like the Xenants. We do have a benefactive thematic affix, and also in the past I've contemplating using a negating verbal operator or an anti-benefactive thematic affix. If we use the anti-benefactive, then maybe "competition" means existing against someone's interest within the context of a contest, and "hostility/attack" means existing against someone's interest through / (by means of) force, and just plain old isting against someone's interest could get a gloss like "interference". I'm quite happy with that. 

Um...speaking of the negating verbal operator, I don't think I ever talked about it in this post. I talked about it a lot in Conlanging I and Conlanging II. The verb CAUSE was also a verbal operator for a while. But I've only used it once in this post implicitly, so maybe it's not all that important - the implicit use was saying that taking mean "to cause something to start not existing with a processed object" which in my head looked like [CAUSE "not(EXIST 1 -with 2)"] or something like that, where the quotes are making the enclosed part a perdurant noun, like "loss". Now, however, I'm wondering if I can do without the NOT operator. Like, we could add in opposites for all the thematic suffixes: Anti-benefactive, Anti-possessive, Anti-instrumental, Anti-similative, Anti-pertinitive. They don't sound very useful, but the first two have already showed up so who knows? I had said before that I wasn't super happy with how the thematic suffixes weren't nicely organized, e.g. into antonymic pairs. Maybe adding in the antonyms is exactly what a Xenant would do. Let's think about when they would/could be useful.

* Anti-benefactive: To exist in a manner that is detrimental or opposed to the interests of X. To act in the disinterest of X. To stand as an obstacle in the way of X's achievements. One word English gloss: "versus".
Competition := exist 1 -versus 2 -about/w.r.t. a contest.
* Anti-possessive: To exist without possession of X. Not not have X. One word gloss: "without".
Loss =: exist -initially 1 -without 2.
* Anti-instrumental: To exist without the use of X. To perform through means other than X. One word gloss: "excepting". This isn't great. I don't want adverbial affixes to be glossed with verbs. What else is there? "Notwithstanding", ..."lacking", ...."without" works fine, but I've already used it ...
Coping =: exist -continuously 1 -with pride -excepting success.
Floating = exist -continuously 1 -over 2 -excepting contact.
* Anti-similative: To exist as something other than X. To be unlike X.
...
* Anti-pertinitive: To exist without pertinence to X. To exist exist in a context unrelated to X. To be about something other than X.
...

Kind of dumb. Anothe pair of perdurants I hadn't categorized was (victory | defeat). Things can happen during those, and they're kind of opposites, yeah? Using the anti-benefactive, we could say something like

exist -terminatively 1 -versus 2 about/w.r.t a contest.

exist -terminatively 2 -versus 1 about/w.r.t a contest.

. In the first affixed verb, party 1 has a victory and party 2 has a defeat;  in the second verb, party 2 has a victory and party 1 has a defeat. It's kind of tricky, because the English perdurants happen together/dependently, rather than exclusively, but the ambiguity of who the perdurant is happening to clears up in the Xenants version - they would instead have opposite words like (a victory(-for-the-self)  | a defeat(-for-the-self), which are exclusive.

I had a little trouble categorizing (leisure | work), which seem kind of antonymic. I think leisure is for yourself while work can be for yourself or for someone else or for a project, et cetera, so the antonymy isn't perfect. Also, it's tempting to say that leisure involves pleasure while work involves pain, maybe as the instrumental means, but the feeling that goes with work isn't exactly, pain. We could say that leisure means causing yourself to exist with inactivity for your own sake while work means causing yourself to exist with activity for the sake of something unspecified? But sometimes people do work or leisure compulsively, and "CAUSE" kind of seems like it's suggestive of the telic -Intentionally affix, while compulsive action is should be marked with the -Unwillingly affix. Hm...... Okay, so I don't know exactly how to define leisure and work, but so far the language is capable expressing all the candidates I've come up with, so my failure to categorize (leisure | work) doesn't indicate that the language is lacking, just that my concepts are vague and not perfectly antonymic     

There's another pair of perdurants I had trouble with expressing, but again I don't think it's due to a expressive shortcoming of the language. Forgetting is a perdurant. And the opposite of forgetting is remembering, yeah? But also forgetting is the opposite of learning, isn't it? And if we code forgetting as losing information ,then perceiving is a third way to gain information, so that's kind of antonymic. I think the Xenants would jut have words for the event of "gaining information | losing information)", and then perceiving, learning, and remembering will be manners of gaining information, perhaps expressed as something like (through sense organ | gain through sense organ and thought | gain through memory). 

There were some perdurants pairs that didn't confuse me, like

(division/separation | unification/fusion/merger)
(disconnection/detachment | connection/attachment)

. but then when I got to categorizing the perdurant "multiplication", I saw that "division" had already been used. And the thing is, when objects multiply, they number of objects increases, but when two numbers multiply, the number of numbers decreases. So there are two different multiplication concepts. Xenants should have one pair of perdurants that expresses "become (more | less) numerous", and also a pair for numerical multiplication. And here's the thing! I think the (division/separation | unification/fusion/merger) pair is suitable for numerical multiplication! Five times three is a unification/fusion/merger of five and three! And I'm also fine with some kind of (addition | subtraction) pair that works on sets and also works on numbers. That doesn't feel metaphorical to me, like I'm using one domain to describe another - it just feels right, like the underlying logic/dynamic is common. So the Xenants have words for (multiplication | division) and (addition | subtraction)  that can be used on numbers as well as non-numbers. We're still missing two famous elementary arithmetic operators - (exponentiation | log-taking/logarithmization). It seems like those two only work on numbers, which is a little sad, but I can deal with it.

In previous posts I thought I might express arithmetic with the simulative affix, like "cause 3 and 5 to exist as a product". I think that's still a good option, but the act of doing so is now called a (whatever word Xenants use for the perdurant of ) unification/fusion/merger. Brilliant!

The last pair of polar perdurants I had trouble expressing was (encryption | decryption). I think we can express that by ....

...

:: Categorical Perdurants

...

:: Nominal affixes for systematic names

I've said that the Xenants have ontological phonosthemes for elements and chemicals, but I haven't given any words for elements or chemicals. One way to write elements that I'd considered in the past is to put an affix on a number, like 1-ium is hydrogen and 12-ium is magnesium. This isn't Xenantish because it puts the ontological type in an affix instead of in the root. If we use genitive affixes, we can say "twelve element -of-atomic-number(1)" for magnesium. This is also convenient for naming nuclides/isotopes, which the Xenants need to be able to talk about since they're radiotrophs. For example, "ninety-two two-hundred-thirty-five element -atomic-number(1) -mass-number(2)" is a systematic way of naming the primordial uranium nuclide on their planet. The Xenants can also  have short non-systematic names for elements, which speciate the element phonostheme in the usual way, just like how English calls element 8 "acid maker" and element 19 "pot ash element" and element 77 "purple element" because of its color in a flame test.

I don't have any strong opinions about how to name chemicals systematically yet. Maybe Xenants use something not so dissimilar from SMILES, the simplified molecular-input line-entry system, but less abbreviated and more regular.

...

:: Hard Words and Soft Roles

There are a few hundred more words that I want to include in the language, and some of them are quite hard to categorize ontologically, maybe because they're roles rather than sortal concepts. Not all, maybe not even most, but some. The page response for me is really latent as I right this, maybe because this post is too large? I think I'll make a new post because the lag is kind of unbearable. See you in the next one, sweetness.

:: Writing System

Maybe Xenants burrow into rock and write in spirals around the tunnels that they dig. To read more of a story, you have to dig deeper. Also, a reader can anticipate plot changes by smelling the pheromones left by previous readers. There's a surprising part up ahead!

...

:: Definitional Aphorisms

:: Babel Story

Cosmopolitan Plant Genera

I want a list of extant plant genera that were present on every continent, except Antarctica, prior to the rise of anatomically modern humans, ~100k years ago. I think that list probably includes.... 

Well, firstly, there were mosses and ferns that evolved in the Devonian, Carboniferous, and Permian eras:
Cystopteris (bladderfern)
Equisetum (horsetail fern)
Isoetes (quillwort)
Lycopodiella (bog clubmoss)
Lycopodium (common clubmoss)
Pteridium (bracken fern)
Selaginella (spikemoss)
There were other plants in those eras like scale trees and seed ferns, but they're extinct now and this list isn't for quitters. Also, there were some moss and fern plants before the Devonian era, but I think they're all extinct.

Next are some much more recently evolved plant genera for which the internet claims at least one species is native to every continent but Antarctica:
Ilex (holly)
Euphorbia (spurge)
Ranunculus (buttercup)
Typha (bulrush/cattail)
Zanthoxylum (prickly ash)
.
Why are they on all of the continents if they evolved after the continents drifted to their current positions? I dunno. Maybe they're good at spreading their seeds on the wind or the water or bird feet or bee legs or fish poop. 

I'm probably missing some other non-vascular plants in the list, like genera of liverworts and hornworts. (Edit: The liverwort genus Marchantia and the hornwort genus Anthoceros are both cosmopolitan.) Also, it's super weird to me that there aren't any conifers on the list yet. Like, {Abies, Cedrus, Pseudotsuga, Tsuga, Sequoia, Pinus} - none of those are natively cosmopolitan, if I remember correctly. I'm running out of conifers.

Some honorable mentions: Ginkgo and Magnolia and Metasequoia (dawn redwood) are quite ancient plant genera (late Permian to Triassic), and we know from fossils that they used to be distributed all over North America, Europe, and Asia. Maybe they were also found in South America, Africa, and Australia/Oceania, but we just haven't found fossils there yet? Or maybe they weren't. Fun fact, the dawn redwood was only known through fossils until 1944, when we found some in Japan.

Also from the fossil record, I think we can say that Cycads used to be everywhere but Europe. They're like stubby palms, kind of, if you're not familiar. Cycads include several genera, and I don't know them very well, but maybe one genus or another was once cosmopolitan, except for Europe and Antarctica, and might still be, except for Europe and Antarctica, for all I know.

Before written history, but not so far back as the fossil record, I think Berberis (barberry) was everywhere but Australia; Aristolochia (Dutchman's pipe) was everywhere but Australia; and Papaver (poppy) was everywhere but South America. I'd be upset with Australia for not having any normal plants, but then I remember Wollemia. Do you know Wollemia? It's is a genus of coniferous trees that was only known by fossils until 1994, when we found some in Australia. Australia might not have normal plants, but it has some great plants.

Finally, Ephedra is a very old genus, and Ephedra species are currently considered native to every continent but Australia, and a fossil of a plant closely related to Ephedra was once found in Australia ("New ephedroid plant from the Lower Cretaceous Koonwarra Fossil Bed, Victoria, Australia" (Krassilov et al., 1998)), so I'd guess that Ephedra once had a cosmopolitan distribution, and it's still an honorable mention today.

If you have more good suggestions, I'd like to hear them.

The Elemental Tech Tree

A follow-up post to "Distillation Daydreams" where I give some details of my recurring fantasy of recreating human technology on my own, possibly in the distant past or alone on a foreign planet.

Some technologies enable other technologies. Let's talk about the sequence of technologies that give you access to different elements of of the periodic table.

If you're on a planet with life, you're in luck. You can thermally decompose organic matter, like dried plants, in a low oxygen environment to get charcoal. Now you have access to a fairly pure source of carbon; one element down. Charcoal burns well, and you can use it to get high temperatures for roasting and distilling things. Also, if you mix powdered charcoal and mineral oil and then ram it into a tube, you get a carbon paste electrode, which is useful for isolating elements by electrolysis. I think graphite powder works better than charcoal powder, but it's way easier to get charcoal powder, so that's what we're doing. I don't know what you can use besides mineral oil as a more readily available liquid binder. It would be awesomely convenient if you could use triglycerides (found in fats/oils), or wax esters (found in beeswax, carnauba wax, sperm whale "oil"), or resin acids from trees. That's something I need to investigate.

Nitrogen gas is in the air. You have access to it already, on Earth anyway. But it's not super usable in that form. Add in oxygen or hydrogen to get more useful chemicals. For example, you can distill protein to make ammonia gas, NH3, and dissolve it in water to get ammonium hydroxide NH4OH.

If you burn organic things, you get ash and charcoal. If you burn things hotter, you get just ash. Ash is mostly calcium carbonate, CaCO3, also called lime. If you heat the ash up even hotter,- if you roast it - then you can drive off CO2 to get calcium oxide, CaO, also called quick lime. That's not direct access to an element, but it's close, and CaO is a very useful chemical for lots of things including other element extractions. For example, if you have a brine with magnesium ions, like evaporated ocean water, you can add CaO to precipitate magnesium hydroxide, Mg(OH)2. Again, not a pure element but it's progress.

Lime is the main part of ashes, but there can be are other things like calcium chloride and salts of sodium and potassium, and these have much higher solubility in water than lime. The ash of deciduous trees tends to have more potassium salts, especially K2CO3 and some KCl, and the ash of plants that grow in salt water tends to have more sodium salts, especially NaCO3 and some NaCl. You can wash these ashes to dissolve the potassium or sodium salts, evaporate off the water, and then roast to get potassium oxide, K2O, and sodium oxide, Na2O, respectively. You need really high temperatures, but it's possible. Make a kiln, burn a lot of charcoal; you can do it, kid. I believe in you. The oxides will spontaneously react with moisture in the air to form hydroxides, KOH and NaOH, which have a million uses. You can also get elemental iodine from seaweed ash, but there are a lot of steps. We'll talk about that one later.

In the last post on distillation, we talked a lot about getting sulfuric acid from minerals, and how it also enables you to get hydrochloric acid, nitric acid, and phosphoric acid from chloride minerals, nitrate minerals, and phosphate minerals. Chloride minerals are free in oceans and seas. How do you get nitrate minerals? Wellllll.... If you keep peeing on a bed of straw for months, bacteria will turn the ammonia in your urine into nitrate, which will bind to trace alkali/alkaline metal in the straw, or to the ammonia, forming ammonium nitrate. Congratulations, now you have a a form of nitrogen with oxygen instead of hydrogen. Nitrate salts are good oxidizers. They're a useful component of gunpowder, among other things. Calcium nitrate can also form as an efflorescence where manure rests on calcium carbonate. So maybe poop on some ashes or limestone, if you want nitrate salts and you don't want to pee on straw? I don't know if bacteria are a necessary part of the efflorescence process. Maybe you can do it even on a planet that doesn't have the right bacteria.

So now we know how to get nitrate minerals. What about phosphate minerals? Bones and teeth have a little bit of calcium phosphate, but not nearly as much as you might think given how hard they are. Still, you can roast them to drive off the organic molecules as gas and and eventually get phosphate ash. Or you can get lucky and find some apatite in the ground, if you know your rocks.

Rocks also have heavy metal ores. If you crush rocks, you can use density methods to separate out the light silica from heavier metals-bearing ores. There are tons of density separations methods and machines, like gold pans and sluices and shaker tables. You don't even need water generally, although it helps. You can totally gold-pan in the desert. One of my favorite density separation methods is gravity-assisted centrifugal separation, in which you pour sand down a helical slide with a curved bottom, like the plastic slides you might find on lots of children's playgrounds. The lighter sands ride up the curve toward the outside and the heavier sands don't. You might not have access to a helical tube slide if you're in the distant past or on Mars; I'm not saying it's the best option. But there are lots of options. Density separation will give you sand with a high iron content, with lots of iron oxides and hydroxides. You can get iron metal from this, but it's not as easy as you might think. We'll talk about it later.

I've heard that once upon a time, in Roman times and before, you could walk around places with exposed bedrock and find veins of lead, and now you mostly don't because we mined it away. All the lead being mined away might not be a problem if you're in the distant past or on a new planet. If you can find veins of it, then lead is pretty easy to mine. You can even melt lead with a wood fire. Setting a fire against a rock wall and dousing the heated rock wall with water is also a good way to creature stress fractures, if you want to mine other metallic ores and you don't have a pick.

Besides the immediate benefits to mood of having ores and metals, and besides their use in structural members, it's also really nice to have at least two different metals if you want to make a battery, though you can also do it with just lead and lead oxide. At first in this fantasy, the metals aren't going to be alkali/alkaline metals, like Na, Mg, K, Ca, because they're very reactive and hard to separate from oxygen unless you already have batteries for electrochemistry. Also we won't start with aluminum metal, which is also too reactive to isolate from oxygen by roasting. What other metals exist in planetary crusts in appreciable quantities? Besides iron and lead, the most abundant metals that you can get at by roasting are mercury, tin, zinc, and copper. Bismuth, silver, and gold are less common, but were also discovered in ancient times - bismuth a little later than the other two. Arsenic and antimony are metalloids that are also accessible: fairly abundant, low melting points, not too crazy reactive. I don't think that anyone makes batteries from those metalloids directly, but they're useful in alloys. Lead with antimony is particularly good for batteries and bearings and bullets. Arsenic with copper, in-place-of or more often in-addition-to tin, makes a good hard bronze that also casts well.

Once you've got metal ores separated from rocks or river bed sand, it's a normal part of mining operations to heat the metal to drive off oxygen or sulfur. If you've got multiple metals, you can probably separate them a bit by melting point. If you've got an alloy that doesn't want to separate by heat, some metals will dissolve more readily in specific acids than others, e.g. silver can be separated from gold with nitric acid since the former is soluble in nitric acid and the latter is not.

So hopefully one way or another you can isolate some metals of decent crustal abundance. Once you have metals, you can use them to make batteries. The original battery design, the Voltaic pile, had stacks of metal plates (two different metals alternating) in a tube, separated by sheets of cellulose, and covered in salt water NaCl brine as an electrolyte. That's all it takes. And you don't even need the cellulose or the tube. Some pairs of metals work better than others (those with dissimilar Standard Electrode Potentials). Acids also make good electrolytes. And I think bases also make decent electrolytes. Basically, there are lots of options. Batteries are not super duper difficult to make.

One of my heroes, the chemist Humphry Davy, just a few years after the Voltaic pile was invented, made huge batteries to split mineral salts by electrolysis and discovered a ton of new elements. You've lived with batteries all your life and have probably never isolated any those elements. The only thing that separates you from the great Humphry Davy is that you're not making giant batteries and electrocuting rocks. You could probably do it this month, if not today or tomorrow. I could too and I haven't - I'm not trying to be too judge-y. But there's not that much that separates us from great scientists besides our lack of will to do things bigger.

What if the only metal you have is lead? Then maybe you can make a lead acid battery and use carbon paste electrodes for wires. To make a lead acid battery, you will also need lead oxide and sulfuric acid. Lead oxide can be made by heating lead in air, and we've already talked about sulfuric acid in the last post. Here's where I should go into more detail about lead acid battery design: ...

One way or another, let's assume you have batteries now and also conductive wire. Congratulations, you can now do electrochemistry. The first thing you'll want to to do is split water. Now you have hydrogen gas and oxygen gas. Their mixture, oxyhydrogen, is kind of terrifying. It likes to explode and turn back into water. It burns so hot it can melt platinum. It's awesome. If you have access to animals, you might be able to store the two gasses separately in bladders. That's how they used to do it. No balloons, just bladders. There are probably other options. Maybe a leather bag waterproofed with tar or something.

The next thing you'll want to electrolyze is NaCl brine. Do this, and you get sodium hydroxide. NaOH, and chlorine gas, Cl2, alongside some oxygen and hydrogen like before. Chlorine is almost as scary as oxyhydrogen. Chlorine both destroys metal labware and kills people with great facility. If you want an easy source of sodium hydroxide without the chlorine, you can instead electrolyze sodium carbonate brine. But it's a lot easier to get sodium chloride than sodium carbonate, so let's talk about what you can do with the chlorine gas.

Spoilers: you make it into hydrochloric acid. We've already talked about making hydrochloric acid by distilling sulfuric acid with sodium chloride. We're making it again now in a different way because sodium hydroxide produced by electrolysis is really useful, but it produces chlorine gas as a byproduct, and chlorine is horrible. If you're making chlorine gas at scale, you need to do something with it at scale, and hydrochloric acid is a great option. I think PVC is also good for chlorine capture, but we're not ready for that. So, how do we combine hydrogen with chlorine? Bubble both gasses into water. Do it for a long time, like overnight, to saturate the water with those gases and displace dissolved oxygen, since oxygen can foul the reaction. Now shine UV light on it. That's it. They'll combine. It's awesome. I'm not sure what the exact wavelength cutoff is for splitting the Cl2 bond. I've seen a demo where blue light did nothing and another where blue light worked. So ... 400 nm or less, maybe? Ultraviolet light definitely works though. If you have a test tube with a stopper, you can shine a black light on it and the stopper pops out along with a cloud of hydrogen chloride. It's a wonderfully quick reaction once you activate it with EM radiation of right wavelength. It's basically a little canon that makes poison vapor, and it's a good idea to do the reaction in plastic instead of glass to avoid shrapnel. Now, sunlight has UV and I bet you can do the reaction under sunlight. What if you want to make a UV lamp? Is there a primitive way to do that? Not that I know of. Mercury vapor lamps give off UV radiation, but we're not ready to make glassware yet. We'll talk about it later. Probably after we've smelted iron and isolated boron. Anyway, shine a light, get some hydrochloric acid, keep it stopped up until you need it. It's way safer than chlorine gas.

So that's the chlorine taken care of. You know what the NaOH is really good for? You can make salts of fatty acids that are a component of triglyceride fats and oils. The salts are called soap, and the leftover glycerol can be nitrated with nitric acid to make nitroglycerin, which is good for mining, among other things.

Electrolysis can also be used to make small amounts of elemental alkali and alkaline metals, like elemental sodium, potassium, and calcium. They're kind of too reactive to be very useful, I think. Like, sodium metal is good for... heat transfer in nuclear reactors? We're not that far along in the fantasy yet. One alkaline metal that *is* awesome and useful is magnesium. It's a great lightweight structural metal, and it's one of the most abundant metals in the earth's crust and it's also abundant in sea water. Despite its use as a structural metal, it's also really reactive. It has enough reactivity to be awesome and enough passivity to to be useful. I really love magnesium. You know who first isolated it? That's right: Humphry Davy. 

A good way to get magnesium metal is to get magnesium hydroxide by reacting CaO or Ca(OH)2 with magnesium-bearing brine, and then react that with hydrochloric acid to get to get MgCl, and then electrolyze the molten chloride salt. You can also just take magnesium oxide, MgO, and reduce the oxygen off with something that loves oxygen, like elemental silicon or carbon, but we haven't talked about isolating silicon yet and doing it with carbon requires really high temps. Magnesium will show up later when we talk about isolating elemental titanium.

On the subject of reactive structural metals, can we get aluminum by electrolysis? Yes, but it's hard. First you need aluminum oxide. That's the easy part; heat clay with hydrochloric acid, which dissolves alumina (producing aluminum chloride) but not silica. React the aluminum chloride with sodium hydroxide to get aluminum hydroxide and roast that to get aluminum oxide. Here's the hard part: You have to melt aluminum oxide before you electrolyze it, and that requires very high temperatures. You can lower the necessary temperature by using a mineral called cryolite as a flux, but cryolite is terribly rare in nature, and the only real way to get it is to make your own. To make your own, you need hydrofluoric acid, which is one of the scarier chemicals to me - considerably worse than oxyhydrogen gas or chlorine. You can make hydrofluoric acid from fluorine bearing minerals like fluorite and fluoroapatite, but... don't. Don't make hydrofluoric acid. Unless you're a immortal time-travelling cyborg, I guess? But even then, do other things first. See how far you can get with magnesium metal. Instead of using cryolite, I hear you can use some potassium or sodium metal to help with the general effort of electrolyzing molten aluminum oxide. That makes aluminum really really expensive - and still pretty dangerous - but it can be done. Also, you can electrolyze a mixture of alumina and copper to make an aluminum bronze, I hear. Cryolite is the reason that aluminum is now cheap enough that lots of people don't even recycle it, when it used to be more valuable than gold, but cryolite is something you do in a late stage of the Elemental Tech Tree, not as soon as you can. Like, hydrofluoric acid attacks basically all metals besides nickel and nickel alloys, so you should get nickel before you try to make it. And it attacks most rubbers, but butyl rubber has good resistance, so learn to make that first. For plastics, learn to make PVC or polypropylene. Now you have a reaction vessel, rubber gloves, and a storage bottle. This is a boss fight and you need to come equipped. Also, you'll need health potions. Calcium gels like calcium gluconate gel are used topically if you get a burn. They don't do enough. You will still die. Calcium and magnesium antacids are also used internally in the case of burns. They also don't do enough. Calcium gluconate is also used intravenously to treat hydrofluoric acid burns, and if you're tech tree isn't grown enough for aseptic injections, you probably shouldn't fuck with hydrofluoric acid, especially not hot concentrated HF, as it comes when you produce it. Also, where are you going to get these fluorine-bearing minerals? Ha, gotcha. Can't do it. Moving on.

This is next bit is... technically part of the elemental tech tree, I think. You can use mineral acids to turn cellulose into glucose, which you can ferment to make dilute ethanol, which you can concentrate by distillation or freeze-jacking. Ethanol will be useful later for phosphorous extraction.

Isolating iodine from seaweed ash: Seaweed ash contains sodium iodide and potassium iodine, NaI and KI, among other things like calcium carbonate, sodium carbonate, sodium chloride, and magnesium chloride. One way to get iodine out is to react the ash with copper sulfate, forming a copper iodide precipitate. Remove the precipitated copper iodide and heat with sulfuric acid to form copper sulfate again and iodine will come off as condensable vapor. Is there a method that doesn't require copper? I don't know. I'll look into it.

Isolation of phosphorous from phosphate salts: ...

Boron: ...

Bromine: ...

Liquid nitrogen: ...

Noble gasses: ...

I think now would be a good time to talk about making iron and steel, which make other things possible, like arc furnaces to get silica and large scale mining machines to get rarer metals.

...

Titanium: The most commercially important titanium ore is ilmenite (trigonal FeTiO3), which is weakly magnetic. You can pick it up with a strong magnet and then pick out contaminants of iron oxides/hydroxides with a weaker magnetic. Another good titanium ore is titanium oxide, TiO2. It has a ton of different mineral polymorphs, including rutile and anatase. TiO2 isn't too hard to find on earth. It commonly makes up a moderate fraction of igneous and metamorphic rocks, like a few percent by mass. It's often mined from sedimentary sands that were weathered from igneous rocks, but if you don't have a beach full of TiO2 or FeTiO3, you can also crush igneous rocks yourself and sort the sand by density. The main way people get titanium from TiO2 industrially is the Kroll process. First, heat TiO2 in chlorine gas producing liquid TiCl4. You can increase your yield by mixing in some elemental carbon with the TiO2, which grabs onto the oxygen. Next, reduce the TiCl4 with liquid magnesium metal, leaving behind elemental titanium sponge. To make it usable, you then break up the sponge, heat and compress it into ingots, and the forge the ingots into jets, jewelry, and hip joints. If you start with FeTiO3 instead of TiO2, you can try to remove the iron before making TiCl4, perhaps with sulfuric acid, or you can try to remove it afterward in the form of a ferric chloride (FeCl3) contaminant.

Gallium: ...

Distillation Daydreams

I daydream about recreating human technology on my own. I've thought about it basically every damn day for years. I don't know how to stop. Maybe writing about some of it will help? It never has before. Maybe I'm just spreading bad thoughts by writing this. Consider not reading this if you don't want to daydream about recreating human technology on your own, every damn day for the rest of your natural life.

Lots of wonderful things can be made or purified through distillation. Some examples:

* Ethanol. If you let yeast ferment sugars into alcohol, you can concentrate the alcohol. Whiskey, brandy, rum, mezcal, vodka, et cetera. Also, you can let your fermented sugars spoil to get acetic acid and distill that out if you like.

* Essential oils. If you boil water and there are a bunch of flowers or pine chips or other plant material in the way of the steam, the steam can absorb some terpenoid oils, and condensing it down lets you make perfumes. Or home remedies for autism or something? I don't know what foolish people do with essential oils. Just use it for perfume. Or use it to flavor your vodka to make gin or limoncello or vanilla extract. But a little bit goes a really long way.

* Mineral acids. A bunch of sulfate minerals can be heated/roasted to produce sulfur trioxide vapors, which can be condensed in water to produce sulfuric acid. The traditionally used sulfate minerals (vitriols) aren't globally uncommon, but it's not like you're going to find them in quantity in your backyard or a river bed or a roadcut. The vitriol minerals are all sulfate salts of heavy transition metals (copper, iron, cobalt, zinc). The more common sulfate minerals on the earth's crust are evaporite deposits of sulfate salts with light alkaline metal cations. I think gypsum (hydrated calcium sulfate) is the most common sulfate mineral. Alchemists didn't use it to make sulfuric acid, but I've heard of it being done in modern times, so maybe it's just difficult? In the 1970s, the Marchon Chemical Works in Whitehaven, UK made sulfuric acid and calcium silicate by roasting gypsum with siliceous rocks. I think it required very high temperatures., since calcium sulfate and sodium sulfate both have thermal decomposition temperatures around 1100 deg C. Eventually, Marchon gave up on gypsum and switched to roasting elemental sulfur, but still, gypsum is some kind of an option, if not necessarily a good one. Other alkaline sulfate minerals found in evaporite deposits include epsomite (magnesium sulfate), baryte (barium sulfate), and thenardite/mirabilitie (sodium sulfate). Alum minerals (double sulfate salts that have both aluminum and an alkaline metal as cations) are also sulfates, and I think they're intermediately common between alkaline sulfates and transition metal sulfates. I don't know how hard it is to make sulfuric acid out of alum by heating. It's probably easier to make sulfuric acid from most of these by electrolysis than by distillation, but that's not an option in the first stages of my fantasy. Whatever the source, once you have made sulfuric acid, you can mix it with other minerals and then distill the mixture to make new exciting mineral acids .Sulfuric acid plus table salt, distilled, gives hydrochloric acid. Sulfuric acid plus nitrate minerals, distilled, give nitric acid. Glauber figured both of those recipes out. Thanks, Glauber. Sulfuric acid combined with with phosphate minerals gives phosphoric acid. Et cetera. It's a very useful chemical, that sulfuric acid. Also you can make sulfuric acid by roasting elemental sulfur or metal sulfide ores, instead of roasting sulfate minerals, but there are some extra steps. It's called the Lead Chamber Process and you have to control the mixing of three gasses and a sprayed liquid. It's not the easiest way to start out your technology recreation fantasy.

* Wood liquors. If you heat up wood in a low oxygen environment (pyrolysis, pyrolytic decomposition, destructive distillation), then gasses and vapors come off and you're left with charcoal. If you condense the vapors, you first get pyroligneous acid, which, besides water, is mostly made of acetic acid and methanol, and a decent amount of acetone. I've read that the distillation of cellulose gives acetic acid and the distillation of lignin gives methanol. Super interesting if true, right? But maybe a little misleading in its simplicity; cellulose is a polymer made of glucose monomers. When you heat it up to thermal degradation in a low-oxygen environment, acetic acid might be the first volatile to distill over, but lots of caramel-like stuff with a higher boiling point is being made in the pot. Likewise, lignin is an irregular polymer made of aromatic alcohols, and methanol might be the first and the main volatile given off by the pyrolysis of lignin, but it's not the only one. Anyway, acetic acid, methanol, and acetone all have some uses, and you can separate them out by fractional distillation (i.e. redistill the mixture with more precise temperature control and with a taller column in the still to encourage reflux). This whole post came about because I was looking into low-tech ways to get methanol for use in biodiesel transesterification. In addition to those three components, your pyroligneous acid will contain a million other random smoky caramelly tar-like organic contaminants in small amounts: formic acid, furfural, pyridine, methyl ethyl ketone, the list just goes on and on. As you keep heating up the wood, tar will start to come over with and then after the pyroligneous acid. Or I guess it could all come over at the same time, if you heat things up quickly or if your still doesn't have any real height for refluxing. If you let the distillation products cool and settle, the tar will be the viscous part at the bottom and the pyroligneous acid will be the runny part at the top. Tar is another wood liquor with some uses - like preserving ship timbers from rot -  although I mostly think of it as contaminant when trying to distill more valuable things. Similarly to distilling wood, if you distill pine resin, you can get turpentine.

* Ammonia. Lots of organic matter has amino acids. Animal tissues in particular have a lot. Amino acids can be distilled to make ammonia gas. Condense it in water and you've got ammonium hydroxide. It's good for cleaning, it's good as fertilizer, it's a decent refrigerant, and it can be used to make hydrazine rocket fuel. And ammonium hydroxide is a convenient base in chemistry and it's in hair dye and other stuff. Lots of uses.

* Low boiling point metals. Some metal can be boiled/vaporized pretty easily and separated from their ores that way. Mercury and lead are pretty easy to distill off of their ores, for example. And at higher temperatures,  you can do more metals. It's pretty cool.

* Petrochemicals. If you have crude oil, which some places in the world do, you can distill it to separate out hydrocarbons by boiling point. Now you can use hexane for acid-base extractions or make candles out of paraffin wax or other things. I hear gasoline has some uses. You can make stuff that like. Petrochemicals can also be made by the Fischer-Tropsch process, although that's not something we do till a much later stage in the tech fantasy.

So there are all these amazing things you can get by heating up plants and rocks and animal tissue in a vessel and condensing the vapors. But how do you do actually distill those things?

"You buy a laboratory glassware distillation apparatus online." No. We're recreating technology on Mars or in the distant past or something. You don't get a glassware supplier. 

"You buy a... copper still online?" Still not quire there.

"You make your own copper still!" Much better! But I'm not very good at metal working, and also, where are we getting all this copper from? Have you ever mined copper? Do you know where to mine  copper? That's not a real plan. 

Honestly, it is a start of a plan. We could make aqueducts to hush the loam from the countryside, revealing bedrock, and then melt ores by fire-setting, maybe. Or we could crush rocks down by a river with a waterwheel and a hammer on a cam follower and then separate out the crushed rock according to density by gravity methods, and in that way find some heavy metals. I don't mean to say that mining isn't possible. But it's not easy and you don't have any guarantee of what you'll find in what places, and it's not usually one of the early steps in my primitive technology daydreams.

The low-tech way to distill things on your own, without a global economy that can extract copper from distant lands and transport it to you, is to make a still from clay. Clay is easy to find, in the distant past, or in your backyard, or even on Mars - all the usual daydream locations - and it's moderately easy to work in a primitive setting, especially compared to metal or glass.

It's a little dangerous to distill alcohol in clay, because the clay could shatter and now you're suddenly throwing alcohol on a fire in an enclosed space, but people in Mexico still make mezcal with clay plots; it's not an insurmountable risk. And it's not like distilling alcohol in metal or glass is without risk. 

The old-timey names for a still are an alembic or a retort, depending on whether it's made from multiple pieces or just one. If you use clay to make a still, and your still has more then one piece to it, with the pieces just stuck together through friction fitting - like a lid on a pot - then the still probably won't be airtight, and your vapors will come out the cracks instead of condensing where you want them. One ancient solution for this is to use dough to plug up holes. It cooks into a horrible little papery hardtack biscuit whish stops gases and vapors from escaping for a while. If your dough-plugs shrink too much as they cook and dry, you can just put on another rolled up snake of dough to plug the new gap. I've done that before with a metal still that was venting steam and I don't really ever want to do it again. What are some other options?

One option is to use a still that only has one piece: a retort. It's a kind of teardrop shape, and the tip bends over and away from the bowl at the bottom to form a condenser tube. Retorts are cool. Hard to clean out though. Kind of hard to fill with solids like animal tissue or woodchips too. I think there's another option.

What we have illustrated here above is a two-piece alembic. The only joint at which we need to stop gasses from venting out is the one where the conical upper piece meets the groove of the lower bowl. Here, the groove is filled with water to help with sealing; I think that's important. You could also put more things in the groove, if you wanted. Like sand, maybe. Or dough snakes. Pine pitch. I don't know. But I think a two-piece alembic makes cleaning and charging the vessel a lot easier than in the case of a retort, and maybe its single joint is a manageable challenge to engineer around, especially if you have a groove full of water, rather than just a friction fit between a pot and a lid. I suppose you could also put grooves on the upper piece and thereby make a little serpentine maze of traps. I don't thing that would help much, but maybe.

Some of the cool distillation products listed above are gasses at standard temperature and pressure and they need to be dissolved in water to make them easily storable and usable, like SO3 becoming sulfuric acid in water or NH3 becoming ammonium hydroxide in water. But if the condenser tube is leading into a container of water, and the seals of the groove also contain water, is there any reason to think that the valuable gas will go in the water that we want, instead of out the side? I don't really know. My hope is that, for example, water can only hold so much ammonia gas, and so the little bit of water in the groove will quickly become saturated, and then the larger amount of water under the condenser tube (not pictured) could keep collecting ammonia gas, and the gas wouldn't start escaping from the system until both reservoirs of water are saturated. I don't know if that's how gas actually works. If that doesn't work, I guess dough snakes are fine. I'd still probably try water and sand first. Or maybe use some wet unfired clay as a plug, in addition to water.

Wait a second, that's not how gas woks at all. You can bubble carbon dioxide though water for a long time and barely increase the carbonic acid content.

This leaves me with a few things I need to figure out to advance the narrative of my primitive technology fantasy, as it pertains to distillation, in the early stages of the fantasy before I've built up resources I need for metal- and glass-working. 

1) I need to prove to my satisfaction that I can make and fire a two-piece clay alembic with a groove for water sealing.
2)  I need to learn more about making sulfuric acid by distillation, either from common evaporite minerals like gypsum, or from elemental sulfur or sulfide minerals. I wouldn't be surprised if there was a good technique that I'm just not seeing in the historical literature or patent literature - maybe something that's too expensive for industry but it works at lower temperatures than the Marchon system.
3) I need to learn more about how gasses work, with respect to, like, partial vapor pressures and solubility and things. I've tried before and it's so boring it just passes between my ears, but maybe having a project on which to use the knowledge will help.
.
Another option that is basically materially equivalent to a clay alembic sealed with uncured clay, but on a larger scale, is to make a kiln out of fired clay bricks and seal it up with mud and straw. You'd still have to figure out a condenser design though. Maybe you could have a side chamber a ways away from the heated portion for liquids to condense or for gasses to dissolve into water. Or, I've heard that if you have a sloping hole out from the bottom of a a kiln where you distill wood, you can get pyroligneous acid and tar to just flow out on the floor. Maybe that works for other some things too. Probably not ammonia and SO3, because they have to be dissolved in water. But maybe some things. Or just make a ceramic tube and put it at the top of the kiln instead of the bottom. Lots of mud to seal, a tube leading into water, done.

...

Isolating Chlorella

I like microalgae. I've been culturing them for more than two years now, but not any fresh-water varieties yet. One of the coolest of the fresh water microalgae is Chlorella, and I just learned that it grows wild basically everywhere that has fresh water, and even in topsoil. So I'm going to try harvesting it from the wild! We've talked about the prokaryotic cyanobacterium Spirulina a lot on this blog. Chlorella isn't like that. It's a unicellular eukaryote with organelles such as a nucleus, a chloroplast, and a mitochondrion. It's what Melvin Calvin, of the Calvin cycle, and Otto Warburg (another towering Nobel laureate, more famous in oncology) experimented on to understand photosynthesis. It's a cool alga.

Actually, harvesting should be the easy part; it's not hard to grow green goo in a jar. What I really want to do is isolate it from other green goo. That means I need to be able to tell one kind of green goo apart from another. So I'm going to get a nicer microscope, because at low to medium resolution a lot of microalgae just look like green circles (they're "coccoid"); some genera can be identified based on larger features like colony shape, but I don't want to rely on just that. I'm going to learn about the distinguishing morphological features of freshwater microalgae, which might be really tiny features like the structure of the chloroplasts. And I'm going to do my best to find Chlorella and grow a sample of only Chlorella. Join me, won't you?

First up, microalgae come from multiple biological kingdoms. Blue-green algae are another name for cyanobacteria. They're prokaryotes with a very simple cell structure and no organelles. Green algae in contrast are eukaryotes, and in particular they're in the plant kingdom, although the single celled ones aren't much like garden plants. A bunch of other microalgae are diatoms, which are unicellular things with silica membranes. Diatoms are are often categorized as protists, which is barely a kingdom. 
The whole protist category is a junk drawer of weird stuff that isn't quite plant, fungus, or animal, but different protists might be more closely related to one of those three groups than to another protist. I'm planning to mostly avoid talking about diatom microalgae in this post, but maybe they'll be an important part of freshwater aquatic systems and I won't have the choice. From what I've read so far, the most common genera that include freshwater diatom species are probably Achnanthes, Amphora, Cymbella, Encyonema, Eunotia, Fragilaria, Gomphonema, Hantzschia, Navicula, Nitzschia, Pinnularia, Planothidium, Sellaphora, Stauroneis, and Surirella.

There are endless freshwater microalgae, but I think the most common genera that I'll have to be able to distinguish from Chlorella (plant) are:

* Microcystis - Cyanobacterium
* Scenedesmus - Plant
* Anabaena - Cyanobacterium
* Oscillatoria/Planktothrix - Cyanobacterium
* Aphanizomenon - Cyanobacterium

Those are the microalgae most commonly referenced in the literature on algae blooms in lakes. I think Nostoc (cyanobacterium) is also pretty common, and that one can grow aquatically or terrestrially, but maybe it doesn't form algal blooms, so it gets mentioned in different literature. Nostoc can also be a photosynthetic symbiont (a photobiont) of lichen colonies, and maybe I should be on the lookout for other lichen photobionts, like Trebouxia, Trentepohlia, Asterochloris, Rhizonema. I think some of them aren't really found as free-living organisms outside of lichen colonies, but if Nostoc is, then maybe some of the others are too. And probably I'll have to learn dozens more microalgae. As the great Delftish microbiologist Lourens Baas-Becking said, but in Dutch, "everything is everywhere"; I'm not going to be able to avoid also learning about all the slightly less common things like Chlamydomonas (plant), Cylindrospermopsis (cyanobacterium), Micrasterias (plant), Monoraphidium (plant), Dolichospermum (cyanobacterium), Lyngbya (cyanobacterium), Synechococcus (cyanobacterium), Raphidocelis (plant), and Pediastrum (plant). I'll have to learn it all. 

As I acquire wild samples and microscope images, I'm going to post them here and discuss what I think they might be, and also chronicle my isolation procedures. Can I use gelatin instead of agar for immobilization? Can I dissolve salts from pulverized rocks to make an effective growth medium or do I need something more precisely formulated like Bold's basal medium? How terrible do these things smell? All this and more in the coming months.

:: The Microscope.

You know microscopes? Pioneered by the great Delftish microbiologist van Leeuwenhoek? We'll need one of those. People on microscope forums say that the popular models on Amazon, like AmScope, OMAX, and Swift are all kind of crummy, and that it's better to buy a used Olympus, Leica, Zeiss, or Nikon microscope. I thought it was a little weird that they were recommending used ones. Do those companies not make new microscopes anymore? They totally do, but the websites for Olympus, Leica, Zeiss, and Nikon do not make it easy to buy or even find a price for new scopes. Maybe they mainly cater to bulk purchasers like universities? So Ebay it is. I'm fine with that.

YouTube has some pretty nice microalgae videos taken through a Leica ATC 2000, with moderate organelle resolution, and those are only like $150 to $250 on eBay, which is a lot cheaper than some of the supposedly crummy Amazon brand models. But the YouTube videos of microalgae from the supposedly crummy Amazon models seem pretty comparable? Let's trust the forums for a moment and keep looking for Olympus, Leica, Zeiss, or Nikon.

I think Leica ATC 2000 uses phase contrast to make sharper images, which can make kind of ugly halos of light around things, but it's still better than not having it, and the videos are honestly pretty cool. If you go a couple hundred or thousand dollars more expensive, you can get microscopes that use differential interference contrast (DIC), which has no halos, and it gives really nice pseudo-3D relief images. Technically, the two contrast techniques are showing different information (path length magnitude versus path length gradient), so they're kind of complementary. I think you can use either one to see fine cellular details like gas vesicles. I've definitely seen some DIC images where you can see amazing organelle structure, but I've also seen ones that kind of just seem to show surface detail, while phase contrast looks more like a somewhat blurry X-ray right through. So I'm not sure if I want DIC or not. It's definitely going to look amazing, but it might be worse for identification if I can't see organelles inside. Most DIC scopes are thousands of dollars, which would make up my mind handily, but there's one on eBay right now for $500. *intense hand wringing* You know, what? I don't believe it. All the DICs are thousands of dollars. There's something wrong about that $500 listing. I'm just going to get a phase contrast microscope. Now I need to figure out why some Leica listings differ by $100 for what appears to be the same model. Ah, some of them don't turn on. They're being sold for parts. It's been a while since I did circuit repair, and I was never very good. I'll just buy a working one.

Hm, eBay is making this more difficult than it should be... Screw it, I don't believe the forums. I'm getting a new one. One with lots of features. AmScope T490B-DK. No regrets. Should be here next week. That gives me some time to get samples.

Oh, butt. Maybe some regrets. The AmScope I bought doesn't have phase contrast. Maybe I should do an interlude about .... histological staining? I feel like I might to need learn a lot about staining in order to resolve cytological features anywhere near as well as I would get with a phase contrast microscope. Hopefully the one I get can be upgraded? Eventually. No rush.

:: The Growth Medium

When growing things in a lab, it's nice to have a well defined food for your algae, but Chlorella really isn't too picky in where it lives and what it eats. From Adaptation to Aquatic and Terrestrial Environments in Chlorella vulgaris (Chlorophyta):

Microalgae in the genus Chlorella (Trebouxiophyceae) are found in almost all geographic regions. The genus comprises species in freshwater lakes, soil, marine, brackish and terrestrial habitats, and some species are also symbionts of lichens, protozoa and invertebrates (Luo et al., 2010; Bock et al., 2011; Darienko et al., 2015).

Two common laboratory growth media for cultivating microalgae are Bold's basal medium and BG-11 medium. Recipes like those mostly just have a bunch of mineral salts dissolved in distilled water. Among the cations of the salts, all of the common alkali metals are represented (Na, K, Ca, Mg) and the anions include nitrate, phosphate, sulfate, chloride, and usually some amount of borate. Then there are usually tiny amounts of some heavy metal salts with cations of iron, zinc, copper, manganese, cobalt, and molybdenum. Also a chelating agent called EDTA is commonly used. I don't know why.

Buying, measuring, and combining all of those salts sounds like a lot of work when Chlorella grows wild in so many environments - including environments that probably have zero EDTA and much lower concentrations of heavy metals. I'm pretty sure you can just use, like, a handful of loamy soil in tap water and you'll have something suitable for Chlorella.

I want to try something a little in-between those two routes in terms of effort. A paper from 1987, "An inexpensive inorganic medium for the mass cultivation of freshwater microalgae", gives a growth medium called DS medium that starts with 90% distilled water and 10% ocean water. This provides all of the alkali metals (Na, K, Ca, Mg) and also chlorides, sulfates, and borates. All they have to add is phosphate, nitrate, and trace heavy elements. I don't live by the ocean, but I do live by rocks, and the salt in the ocean comes from the erosion of continental rocks. Strickler's 3rd Law of GeoFantasy: "The earth breaks what it makes and puts it in the ocean." So how about I try that? I'll pulverize some rocks into powder and maybe some small portion will be immediately dissolvable into water without any acidification (which would simulate weathering over long times scales by slightly acidic rain). Or if it does need acidification, I can use some phosphoric acid and some nitric acid, and now those anions are covered also. I think this is an excellent idea. It can be done inorganically, which limits sample contamination, it can be done far from oceans, which is convenient if you're in, say, Wisconsin or a Mars habitat, it doesn't require any EDTA chelating agent. There is a question in my mind of how much rock I'm going to have to pulverize to get appreciably quantities of growth medium with a salinity 10% that of ocean water, but that's a problem for near-future me. If you've followed my writing about growing Spirulina on Mars, you also know that there's an easy way to get phosphates and nitrates for growing algae, if you don't have the corresponding mineral acids handy.

Oh! After a little reflection, it seems to me that pulverizing rocks to extract soluble salts is perhaps a rather high effort option. It's still absolutely something I'm going to do; I need to know if it's viable. But first, I'm going to try something else. I've already got a nice abiological growth medium for my Spirulina with lots of mineral salts, including nitrates and phosphates and carbonates. Spirulina likes salty brine lakes, comparable in salinity to ocean water I think but with much lower acidity (much higher pH). So my first idea for a low effort abiological culture medium is start with spirulina medium, raise the acidity a little so that we're closer to ocean water, and then dilute with 9 parts distilled water to 1 part fake ocean water, just like the DS medium. That's probably not a great option for you kids following along at home, but it's what I'm going to do till I've had a day to crush rocks.

:: Our Beloved Protagonist

Chlorella was discovered in a pond in the Netherlands by the great Delftish microbiologist Martinus Willem Beijerinck, who also discovered viruses, nitrogen fixation, and sulfate reducing bacteria, among many other things. One of the things that makes Chlorella interesting is its potential use as a food source. It's a complete a protein, and it makes bioavailable vitamin B12 (but possibly only if it already has a source of non-bioavailable pseudo-B12), and its makes triglyceride oil rich in omega-3 fatty acids. I think it could be a very useful passenger on long-term space missions, where it is would also be useful for its ability to improve air through photosynthetic carbon capture, just as plants do.

What does it look like? What are its identifying morphological characteristics? It's green. It's round. Each cell has a size between 2 and 10 μm (mirometers, microns). Unfortunately, the common and hepatotoxic alga Microcystis is also green and round, and it has a size between 2 and 7 μm. Microcystis also happens to be a cyanobacterium, so no internal organelles. Chlorella doesn't have any flagella for moving. I'm pretty sure it's also non-motile, but some unicellular organisms without flagella can move a little bit anyways, and we're still figuring out how in some cases, and I'm not sure if Chlorella is one of them. One method used by bacteria is called twitching and it involves type IV pili, which are protein fibers on the surfaces of some bacteria. Another strange method of bacterial motion is gliding, which involves gliding motors in the cell membrane pushing against adhesion complexes? Chlorella isn't a bacteria, and as for as I know, Chlorella can't do either of those. In microscopy videos, it certainly looks pretty damn immotile, except that its contents expand and push against each other a little when it reproduces. I've heard that Microcystis can twitch or glide, but I haven't seen a video of it moving. Sources often remark on Chlorella's chloroplasts as distinguishing features. One source claims that each cell has a single cup-shaped chloroplast. Another says its chloroplasts are plate-like or cup-shaped, and doesn't give a count.. When they say cup-shaped, I think they mean that, looking down on the cell, you see a broken circle, like the letter "C". I'm going to be real with you: most high resolution images of Chlorella just look kind of bumpy. It's hard to tell what the bumps are. But even if we can positively say, "these bumps are a cup-shaped chloroplast", I don't know how diagnostic that is. If you google "cup-shaped chloroplast", you'll see that Chlamydomonas more famously has them. Chlamydomonas has a flagellum, so you're not going to confuse it for Chlorella, but the point is, a cup-shaped chloroplast isn't unique. Not super related, but Chlamydomonas with the flagellum, it can grow in snow! So cool. Oh hey, I just saw another source saying that Chlorella has only one chloroplast per cell. I believe it. I just don't know it when I see it. One Chlorella diagram on the web shows two chloropasts, each taking up about half of the cell volume, looking a lot like a yin-yang symbol. That's interesting in that the yin-yang components are kind of cup-shaped, but it doesn't match any images of Chlorella I've seen and also, two chloroplasts? Pretty suspicious.

So at present, to identify Chlorella, the best things we have to go on are 1) small, round and green with no flagellum (which rules out Chlamydomonas) and 2) does have at least one cup-shape chloroplast (which rules out Microcystis, if we can figure from microscope images where the chloroplast is, or isn't in the case of the cyanobacterium).

Some unicellular microalgae form interesting colonies. Small Mycrocystis colonies have individual cells grouped together loosely in a ball of clear mucous, not always directly touching. A larger colony sometimes elongates and looks more like a fuzzy caterpillar, or just a fuzzy ball if it doesn't elongate. What about Chlorella? One source says it can produce colonies up to 64 cells. Some cyanobacterial microalgae, when they form colonies, will have a few cells that fix atmospheric nitrogen for the colony instead of performing photosynthesis, and they look different (bigger usually, I think) and the nitrogen cells are called heterocysts. I don't think Chlorella can do that. If you see a colony with heterocysts, you haven't isolated Chlorella. So what's the point of forming a colony for Chlorella? It's not for division of labor with heterocysts, and it's not for mating, because Chlorella reproduces asexually. Does it excrete an extra-cellular polymeric substance that becomes more useful when all the colony chips in?

...

:: Unialgal And Axenic Isolation

The basic procedure for isolating an alga is to spread an environmental water sample very thinly on an immobilizing substrate like agar in a Petri dish, perhaps by dipping a wire loop or needle into the sample and then streaking it across the dish. Next, incubate for a few days and, finally, examine your handiwork. If you spread very small quantities thinly on the dish, then each little region on the dish will hopefully have just one species of alga. Now it's unialgal. Congrats. But what if there's one alga and also something else on there that isn't an alga? The easiest thing you can do repeat the process until you get just one species. If that works, then your culture is "axenic". Double congrats. What if, like, all of your chlorella cells have infections? Maybe each one contains an even tinier vampire bacterium called Vampirovibrio. What do you do now? I'm not sure. Maybe hope that you find a chemical or genomic treatment that attacks the parasite and not the host. Or maybe "every cell is infected" isn't a real problem. Like, when a Chlorella cell reproduces, I don't think the daughter cells would all have the infection. If you make a streak plate, one of your regions will eventually be pure, just by statistical coincidence. It's not a real problem. 

Or rather it wouldn't be a problem if I were trying to get an axenic culture. I'll do my best to limit contamination in my samples, but you need some high grade aseptic laboratory equipment and procedures to get rid of all contaminants and keep them away. Everything I just said can be done with great fastidiousness to avoid contamination, like by wearing surgical gloves, and disinfecting surfaces before working (including the gloves), and working in a laminar flow hood, and sterilizing your sampling wire under a spirit flame, and sealing your Petri dishes with thermoplastic parafilm, and incubating your dishes in a sealed chamber incubator, et cetera. But even with all of that, it's still really pretty hard to get and maintain an axenic culture. So I'll settle for a unialgal culture.

I'm going to try some other immobilizing agents besides agar to start, using materials at hand. I've heard gelatin works pretty well so long as the organisms in your sample don't digest it, but that this is a common problem. I've also heard of mixed success with either xanthan gum or pectin as an immobilizing agent for making streak plates. And as long as we're trying stuff, how about a gel made from polyethylene glycol and calcium acetate. That sounds interesting to me. Chlorella can eat acetate, but I still want to try. Oh! Silica gel! Absolutely yes, going to do silica gel.

How do you make silica gel? Start with silica, for example, silica beads from desiccant packets, or bulk silica beads that they sell in some craft stores for drying out flowers. If you use desiccant packets, probably don't use the color changing variety since they can have cobalt chloride as an indicator of hydration, which is a little cancerous. Combine your silica with water and sodium hydroxide to make a basic solution of sodium silicate. Now add in sulfuric acid dropwise. Lower the pH down around 6 and the let it stand; a fairly opaque gel will form throughout the whole container. Or maybe you can stir it a little bit to prevent localized coagulation, but don't add more acid. Use an inorganic nutrient medium instead of water and now you have a immobilizing nutrient gel which can support microbial life while halting diffusion and convection currents. And you can use other acids like hydrochloric acid if you want. Then your nutrient medium will be a little enriched with sodium chloride instead of sodium sulfate. I feel like phosphoric acid or nitric acid would be ever better, nutritionally. Or a mix? I'll try some stuff and let you know how it goes.

Another trick to help with isolation, besides the streak plate with an inorganic immobilizing agent and the aseptic work space, is serial dilution. Instead of taking straight from a sample of pond water when streaking, you can dilute your pond sample by putting just a little it into some growth medium. And then you dilute that by putting it into a bunch of batches of growth medium. And then you dilute that. And then you do streak plates for a bunch of your dilute samples, and hopefully one of them will end up free of whatever molds and non-photoautotrophic bacteria were in the original sample. Or if they're not gone, then at least you will have diluted the soil-like carbon sources that they feed on, so that the photosynthetic guys can dominant the sample, since they can feed on CO2 gas and whatever carbonate or bicarbonate anions if you have in your growth medium. Serial dilution is especially a good idea for soil samples, since they're full of organic carbon and molds and bacteria.

When you're all done isolating a sample, one easy test for the presence of fungi and non-photosynthetic bacteria is to take a little out and add a complex carbon source, like some sugar. It won't take long to spoil if they're in there.

:: Diagnostic Antibiotics

If more than half of the common microalgae are cyanobacteria, can we just use an antibacterial to double our chances of isolating Chlorella? Maybe. Table 2 on page 4 of the paper "Combined Effects of Sulfamethoxazole and Erythromycin on a Freshwater Microalga" (Zhang et al., 2021) shows that Chlorella is pretty resistant to sulfamethoxazole, an antibiotic commonly prescribed for humans, in comparison to some other cyanobacteria and microalgae plants. 

I'm absolutely going to get some aquarium grade antibiotics and do some tests. This sounds awesome. It might sounds less awesome to you if you've ever had to take antibiotics orally, and you know that they mostly smell and taste like sewage. I'm not worried about the smell. I think pond scum is more likely to smell bad if I don't treat it with antibiotics than if I do, and I was already prepared for that experience. I still think this will be a great.

What's even left after you get rid of the cyanobacteria and the diatoms?

The class of Chlorella is called Chlorophyceae. Its members seem to be fairly common. Famous genera include Botryococcus, Chlamydomonas, Chlorella, Dunaliella, Haematococcus, and Scenedesmus. Some other honorable mentions are (Tetraspora, Pediastrum, Hydrodictyon, Neochloris, Tetraselmis, Nannochloropsis).

There's another class of freshwater unicellular green algae called Charophyceae that's more closely related to land plants than Chlorophyceae, but also I think a lot rarer. It's most famous genera are probably (Closterium, Cosmarium, Desmidium, Micrasterias, Spirotaenia, Spirogyra, Staurastrum) with (Actinotaenium, Euastrum, Gonatozygon, Hyalotheca, Netrium, Pleurotaenium, Spondylosium, Xanthidium) coming in second tier.

I'm not sure if any of the others families matter. I'm still reading.

A lot of the genera above aren't coccoid, or they form filamentous colonies, or they have flagella, and none of those things are true of Chlorella. I think if I can filter rout cyanobacteria and diatoms and fungi and non-photosynthetic bacteria, and if Chlorella is as cosmopolitan as reports lead me to believe, then I have a good chance of identifying and isolating it.

I think, to avoid breeding antibiotic resistant bacteria, I'm going to use antibiotics diagnostically, but not for isolation. I'll isolate species with streak plates, and then rule out ones that are probably not Chlorella  by taking a little out and testing their susceptibility to antibiotics.

Most antibiotics are used as anti-bacterials, and if you want to get rid of yeasts and molds, then you use specialized antifungals. I don't anticipate needing to use antifungals diagnostically, but there are some green spherical yeasts and maybe I should be prepared for those.

Ooh, just found a paper called "The use of antibiotics to obtain axenic cultures of algae" which even talks about Chlorella. Things are looking up. And it even talks about common Chlorella contaminants, which is super important to me because I think Chlorella might need a source of pseudo-B12 (like a bacterial contaminant) in order to make B12 that's bioavailable for itself and for humans.

:: Vitamin B12 tho?

I think it's funny that almost all of the pioneering microbiologists I've mentioned have been Dutch dudes who lived or at least studied in Delft. There's one more I'd like to make a reference to, Albert Kluyver, and this will probably be the section.

Vitamin B12 is only made by archaea and bacteria, I believe. Chlorella cultures contain B12. Chlorella is a eukaryote, not an archaeon or bacterium. What gives?

The paper has the explanation, but the title completely skips the interesting bit. Bacteria make their own B12, then Chlorella converts it into a form of B12 that it and we can use. That paper talks about Chlorella combining bacterial B12 with a chemical called DMB on the lower ligand. Where does *that* cone from? We just found out in 2015.

So at present, my prediction is that a pure sample of Chlorella, a unialgal axenic culture, can't make B12, and all chlorella cultures that contain B12 have bacterial contamination. It would be cooler if Chlorella had absorbed a cyanobacterium and used it as a B12 organelle, or if it was making pseudo-B12 from scratch following cross-species gene transfer, but not this time.

What are the likely contaminants? The cool answer would be Chlorella's vampire. It's a cyanobacterium that gave up on photosynthesis in preference to predation. So cool. As a bacterium associated with Chlorella, might it be Chlorella's source of pseudo-cobalamin? The literature doesn't say anything about it, and it's the sort of thing we'd investigate and publish, so probably not.

One likely contaminant is Thaumarchaeota (aka Nitrososphaerota), a globally distributed archaeon phylum found widely in aquatic environments. It's everywhere, but especially prevalent relative to other B12 producers in colder and deeper water columns

Chlorella can also live terrestrially though. Where does it get its pseudo-B12 when it's on land? Maybe the same place:"Thaumarchaeota are the dominant archaea in most soil systems where they constitute up to 5% of all prokaryotes"(from Ammonia-Oxidising Archaea – Physiology, Ecology and Evolution).

So it seems likely that Chlorella associates with different Thaumarchaeota in the wild, not to rule out associations with cyanobacteria or proteobacteria or anything else. But wait, Thaumarchaeota might not be the contaminant in laboratory cultures, because... I think they're all huge. They're sometimes called giant archaea and their sizes can be like 13 to 28 microns wide, while Chlorella is like 2 to 10 micron. Hugeness means they're less likely to be a contaminant in lab culture, the same way we don't expect squirrels to be contaminants on account of their size. You'd see them on the streak plate when doing your isolation procedures. In comparison, Chlorella's vampire is ~ 0.6 micron.

But wait, someone found out the common laboratory contaminants in " "The use of antibiotics to obtain axenic cultures of algae"! 

First they reference an older paper:

"A typical large-scale continuous culture of Chlorella sorokiniana Shihira et Krauss for example, was found by Litchfield, Colwell & Prescott (1969) to be heavily infected with Pseudomonas, Acinetobacter, Flavobacterium and Bacillus bacteria, even when grown at the selective temperature of 39 ° C."

Very interesting. In their own experiments,

"The Chlorella cultures yielded an abundance of both yeasts and orange colonies of isolate Type No. 1, it was strange, however, that only from the most complex antibiotic mixture were bacterial colonies visible."

"Yeast-free healthy cultures of Chlorella were obtained with the streptomycinfree antibiotic mixture No. 2. It was surprising that the richer mixture, No. 1, did not eliminate the orange coryneform bacteria; it may have been due to antagonistic effects in the antibiotic mixtures."

So yeasts are common contaminants, but they don't make any form of B12. So let's look into the genera from the referenced older paper (Pseudomonas, Acinetobacter, Flavobacterium, Bacillus) and also the "orange coryneform bacteria" in the present paper.

"Coryneform" just means that the bacteria have a rod-like or club-like shape. I think the orange coryneform bacteria are all gram-positive, which means they take up crystal violet stain and appear purple in the Gram stain test. The reason they take up violet stain is that they have peptidoglycan (amino aid + sugar) polymers in their outer plasma membranes/cell walls. The orange coryneform bacteria include genera of Corynebacterium, Brevibacterium, Dermabacter, Microbacterium, and Cellulomonas. One of those, Brevibacterium, is responsible for the orange color on the rinds of traditionally-made Munster and Limburger cheese, though what you get in the grocery store is probably just colored with annatto made from achiote seeds.

So, now we have a list of bacterial genera that are common contaminants of laboratory Chlorella cultures. Which ones make some kind of cobalamin?

* Pseudomonas: This one can make B12. Pseudomonas denitrificans is even used industrially to produce B12 at scale.
* Acinetobacter: No B12 production that I can find.
* Flavobacterium: Definitely makes B12. The paper "Production of Vitamin B12 by Microorganisms and Its Occurrence in Plant Tissues" (1952) lists nine different species that make B12, but they all have different names now because biologists suck.
* Bacillus: Some species in this genus produce B12. Bacillus megaterium is used industrially to produce B12 at scale. Bacillus badius makes it, as reported in "Utilization of Hydrocarbons and Vitamin B12 Production by Bacillus badiust" (1976). The 1952 paper with the Flavobacteria also lists Bacillus subtilis, but the paper "Microbial production of vitamin B12" (2017) seems to say say no: "Bacillus subtilis lack[s] the genes involved in the cobalamin synthesis pathway after precorrin-2". 

So most of those can  make B12. But I can't find statements that any of the the orange coryneform ones make B12.

* Corynebacterium: I can only find references to  Corynebacterium species that do not produce B12. The genus Corynebacterium includes diphtheria, and also C. glutamicum, which is used to make massive quantities of the amino acids L-glutamate and L-lysine for animal feed, including human feed. 

No positive references yet for any species in the genera Brevibacterium, Dermabacter, Microbacterium, or Cellulomonas that can make B12, but while looking I found to fairly general statements about some things to do make it:

"Vitamin B12 can usually be synthesized by many bacterial species, especially heterotrophic bacteria, and nearly all of the oxygenic photosynthetic cyanobacteria,"

"Nearly all methylotrophic bacteria can produce vitamin B12 at concentrations ranging from 30 to 150 μg/gbiomass [81], with members of the Methylobacterium genus reported to produce B12 at higher concentrations compared to other genera"

So those are interesting.


:: Samples

:: Bioreactors

:: First Contact

...